Cell-free microRNA expression profiles in malignant effusion associated with patient survival in non-small cell lung cancer.
Cell-free microRNA expression profiles in malignant effusion associated with patient survival in non-small cell lung cancer.
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与非小细胞肺癌患者生存相关的恶性积液中的无细胞 MicroRNA 表达谱
DOI：
10.1371/journal.pone.0043268
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Hou Y
中科院分区：
文献类型：
--
作者：
Wang T;Lv M;Shen S;Zhou S;Wang P;Chen Y;Liu B;Yu L;Hou Y
目的研究肿瘤细胞中MicroRNAs （miRNAs）的表达变化，并将其作为肿瘤患者诊断和预后的生物标志物。本研究旨在分析恶性胸腔积液（MPE）中循环miRNAs的表达及其与非小细胞肺癌（NSCLC）患者生存率的关系。方法收集184例非小细胞肺癌合并MPE患者的胸腔积液。采用MiRNA微阵列技术和生物信息学方法对10例不同生存预后的NSCLC患者进行MiRNA表达谱分析。采用定量RT-PCR对184例患者（随机分为训练组和验证组，每组人数相等）进行关联验证。根据mirna的表达特征制定风险评分。收集患者生存期等临床资料进行相关性分析。结果在存活时间较长组和存活时间较短组的恶性积液中，有33种mirna发生了2倍以上的改变，其中5种mirna （miRNA-93、miRNA-100、miRNA-134、miRNA-151和miRNA-345）的水平与总生存率显著相关。在训练和验证队列中，miR-100的高表达和miRNA-93、miRNA-134、miRNA-151和miRNA-345的低表达与较差的生存率相关。与低风险评分的患者相比，高风险评分的患者总体生存率较低。风险评分是患者生存的独立预测因子。结论在非小细胞肺癌MPE中，mirna的表达模式发生了系统性改变。来自积液的5种miRNA特征可以作为肺癌患者总生存期的预测因子。
Objective MicroRNAs (miRNAs) expression is altered in cancer cells, and miRNAs could serve as diagnostic and prognostic biomarker for cancer patients. This study was designed to analyze circulating miRNAs expression in the malignant pleural effusion (MPE) and their association with patient survival in non-small cell lung cancer (NSCLC). Methods Pleural effusion from 184 patients with NSCLC and MPE were collected. MiRNA microarray and bioinformatics interpretation were used to evaluate miRNA expression profiles in 10 NSCLC patients with different survival prognosis. Associations were validated in 184 patients (randomly classified into training and validation set with equal number in each group) using quantitative RT-PCR. Risk scores were formulated based on the expression signature of miRNAs. Clinical data, such as patient survival, were collected for correlation analysis. Results Thirty-three miRNAs were found to be altered more than two-fold by microarray in malignant effusions between longer-survival and shorter-survival groups, and levels of five miRNAs (miRNA-93, miRNA-100, miRNA-134, miRNA-151 and miRNA-345) were significantly associated with overall survival. High expression of miR-100 and low expression of miRNA-93, miRNA-134, miRNA-151 and miRNA-345 were associated with poor survival in both the training and validation cohort. Patients with high risk scores had overall poor survival compared to the patients with low risk scores. Risk score was an independent predictor of patient survival. Conclusions Expression patterns of miRNAs are systematically altered in MPE of patient with NSCLC. The five miRNA signature from the effusion may serve as a predictor for the overall survival of patients with lung cancers.
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影响因子：
64.8
作者：
Lu, J;Getz, G;Golub, TR
通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
11.2
作者：
Iorio, MV;Ferracin, M;Croce, CM
通讯作者：
Croce, CM