Evidence for estrogen receptor in cell nuclei and axon terminals within the lateral habenula of the rat: Regulation during pregnancy
Evidence for estrogen receptor in cell nuclei and axon terminals within the lateral habenula of the rat: Regulation during pregnancy
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大鼠外侧缰核内细胞核和轴突末端雌激素受体的证据：妊娠期间的调节
DOI：
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
J. Morrell
中科院分区：
文献类型：
--
作者：
C. Wagner;A. Silverman;J. Morrell
缰核复合体参与雌性大鼠性行为、母性行为和产后性行为等多种雌激素依赖的生殖行为。虽然已知雌激素在大脑其他区域起作用来调节这些行为，但目前尚不清楚雌激素是否也直接作用于缰核复合体。为了解决这种可能性，我们检查了这一区域雌激素受体(ER)的存在。通过原位杂交、光镜和电子显微镜水平的免疫细胞化学以及类固醇放射自显影，这一分析在不同的实验中进行。外侧缰核(LHb)内的神经元(而不是内侧缰核)表达ER mRNA，在其核中含有ER免疫反应(ER-ir)，并浓缩标记的雌二醇，为外侧缰核存在功能性ER提供了强有力的证据。在LHb内也有含有ER-ir的点状纤维，在电子显微镜水平上，这些纤维部分被证明是轴突和突触前轴突终末。在孕期和产后，LHb细胞核内ER-ir的水平和ER-ir纤维的密度均受到调节，提示LHb对雌激素的敏感性在此期间可能发生改变。综上所述，这些结果表明，LHb可能是一个比以前认为的更具雌激素敏感性的区域，并为ER提供了替代的作用机制。LHb内的内质网可能在LHb参与雌激素依赖的雌性生殖行为中起关键作用。J.Comp.神经。392：330-342,1998。©1998 Wiley-Liss，Inc.
The habenular complex is involved in several estrogen‐dependent reproductive behaviors in female rats, namely, sexual behavior, maternal behavior, and postpartum sexual behavior. Although it is known that estrogen acts in other brain regions to mediate these behaviors, it is not known whether estrogen may also act directly on the habenular complex. To address this possibility, we examined this region for the presence of estrogen receptor (ER). This analysis was carried out in separate experiments by using in situ hybridization, immunocytochemistry at the light and electron microscopic levels, and steroid autoradiography. Neurons within the lateral habenula (LHb), but not the medial habenula, express ER mRNA, contain ER immunoreactivity (ER‐ir) in their nuclei, and concentrate radiolabelled estradiol, providing strong evidence for the presence of functional ER in the lateral habenula. There were also ER‐ir containing punctate fibers within the LHb, which, at the electron microscopic level, in part, proved to be axons and presynaptic axonal terminals. Both the level of ER‐ir in cell nuclei and the density of ER‐ir fibers within the LHb were regulated during the course of pregnancy and the postpartum period, suggesting that the sensitivity of the LHb to estrogen may be altered during this time. Taken together, these results demonstrate that the LHb is likely a more estrogen‐sensitive region than was previously considered, and they suggest alternative mechanisms of action for ER. ER within the LHb may play a critical role in the involvement of the LHb in estrogen‐dependent female reproductive behaviors. J. Comp. Neurol. 392:330–342, 1998. © 1998 Wiley‐Liss, Inc.
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影响因子：
4.8
作者：
J. Blaustein
通讯作者：
J. Blaustein
影响因子：
16.6
作者：
D. Clayton
通讯作者：
D. Clayton
怀孕期间雌性大鼠行为相关大脑区域的雌激素受体免疫反应水平发生变化。
DOI：
10.1016/s0169-328x(96)00141-6
发表时间：
1996
期刊：
Brain research. Molecular brain research
影响因子：
--
作者：
Wagner,CK;Morrell,JI
通讯作者：
Morrell,JI
妊娠期雌性大鼠脑内雌激素受体 mRNA 表达的原位分析
DOI：
10.1016/0169-328x(95)00120-h
发表时间：
1995
期刊：
Brain research. Molecular brain research
影响因子：
--
作者：
Wagner,CK;Morrell,JI
通讯作者：
Morrell,JI
缰核复合体外侧细分的神经元介导大鼠母体行为的激素起始。
影响因子：
1.9
作者：
Corodimas,KP;Rosenblatt,JS;Canfield,ME;Morrell,JI
通讯作者：
Morrell,JI