Pattern recognition of cancer cells using aptamer-conjugated magnetic nanoparticles.
Pattern recognition of cancer cells using aptamer-conjugated magnetic nanoparticles.
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DOI：
10.1021/nn3002328
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发表时间：
2012-05-22
期刊：
影响因子：
17.1
通讯作者：
Tan, Weihong
中科院分区：
文献类型：
--
作者：
Bamrungsap, Suwussa;Chen, Tao;Shukoor, Mohammed Ibrahim;Chen, Zhuo;Sefah, Kwame;Chen, Yan;Tan, Weihong
生物相容性磁性纳米传感器基于分散的磁性纳米颗粒可逆自组装成稳定的纳米组件，已被用作有效的磁弛豫开关（MRSw）来检测分子相互作用。我们首次报道了基于适配体共轭磁性纳米颗粒（ACMNPs）的核磁共振波设计。ACMNPs利用适配体特异性结合靶癌细胞的能力，以及MNPs的大表面积来适应多个适配体结合事件。在250 μL的样品中，ACMNPs最多可检测到10个癌细胞。ACMNPs在细胞混合物和复杂生物培养基（包括胎牛血清、人血浆和全血）中的检测也证明了其特异性和敏感性。此外，通过使用一系列ACMNPs，可以通过模式识别区分各种细胞类型，从而创建细胞分子谱，使临床医生能够在分子和单细胞水平上准确识别癌细胞。
Biocompatible magnetic nanosensors based on reversible self-assembly of dispersed magnetic nanoparticles into stable nanoassemblies have been used as effective magnetic relaxation switches (MRSw) for the detection of molecular interactions. We report, for the first time, the design of MRSw based on aptamer-conjugated magnetic nanoparticles (ACMNPs). The ACMNPs capitalize on the ability of aptamers to specifically bind target cancer cells, as well as the large surface area of MNPs to accommodate multiple aptamer binding events. The ACMNPs can detect as few as 10 cancer cells in 250 μL of sample. The ACMNPs’ specificity and sensitivity are also demonstrated by detection in cell mixtures and complex biological media, including fetal bovine serum (FBS), human plasma, and whole blood. Furthermore, by using an array of ACMNPs, various cell types can be differentiated through pattern recognition, thus creating a cellular molecular profile which will allow clinicians to accurately identify cancer cells at the molecular and single cell level.
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影响因子：
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作者：
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DOI：
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发表时间：
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期刊：
Chemistry (Weinheim an der Bergstrasse, Germany)
影响因子：
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