Nanoparticle-target interactions parallel antibody-protein interactions.
Nanoparticle-target interactions parallel antibody-protein interactions.
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DOI：
10.1021/ac802717c
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发表时间：
2009-05-01
影响因子：
7.4
通讯作者：
Josephson, Lee
中科院分区：
文献类型：
--
作者：
Koh, Isaac;Hong, Rui;Weissleder, Ralph;Josephson, Lee
当磁性粒子与目标分析物结合时，它们可以充当磁弛豫开关(MRSw)，并在分散和聚集状态之间切换，导致其周围水质子的自旋-自旋弛豫时间(T2)发生变化。纳米粒子(NPs，10-100 nm)和微米级粒子(MPS)都被用作核磁共振波谱，用于检测药物、代谢物、寡核苷酸、蛋白质、细菌和哺乳动物细胞。为了更好地了解NPs或MPS与靶标的相互作用，我们利用流感病毒血凝素的标记肽与该标记肽的单抗(抗TAG)之间的反应作为分子识别系统。为了获得不同大小和不同价态的靶标，我们将该标签肽连接到牛血清白蛋白(Mw=65000 Dalton，直径=8 nm)和乳胶球(直径=90 0 nm)上。为了获得不同大小的磁性探针，反标签被连接到40 nm纳米粒子和1μm MPS上。MP和NP探针以类似于溶液中抗体/抗原反应的方式与标记多肽靶标反应，表现出所谓的前地带效应。MPS比NPS对所有类型的靶标都有更高的灵敏度，高价靶标比低价靶标检测得更好。标签/反标签识别系统可用于合成分子靶标和磁性探针的组合，以更全面地了解当探针结合溶液中的靶标时发生的聚集反应以及随之而来的水松弛时间的变化。
Magnetic particles can act as magnetic relaxation switches (MRSw's) when they bind to target analytes, and switch between their dispersed and aggregated states resulting in changes in the spin-spin relaxation time (T2) of their surrounding water protons. Both nanoparticles (NPs, 10-100 nm) and micron-sized particles (MPs) have been employed as MRSw's, to sense drugs, metabolites, oligonucleotides, proteins, bacteria and mammalian cells. To better understand how NPs or MPs interact with targets, we employed as a molecular recognition system the reaction between the Tag peptide of the influenza virus hemagglutinin and a monoclonal antibody to that peptide (anti-Tag). To obtain targets of different size and valency, we attached the Tag peptide to BSA (Mw= 65000 Daltons, diameter = 8 nm) and to Latex spheres (diameter = 900 nm). To obtain magnetic probes of very different sizes, anti-Tag was conjugated to 40 nm NPs and 1 μm MPs. MP and NP probes reacted with Tag peptide targets in a manner similar to antibody/antigen reactions in solution, exhibiting so-called prozone effects. MPs detected all types of targets with higher sensitivity than NPs with targets of higher valency being better detected than those of lower valency. The Tag/anti-tag recognition system can be used to synthesize combinations of molecular targets and magnetic probes, to more fully understand the aggregation reaction that occurs when probes bind targets in solution and the ensuing changes in water relaxation times that result.
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影响因子：
3.3
作者：
Hong, Rui;Cima, Michael J.;Josephson, Lee
通讯作者：
Josephson, Lee
影响因子：
16.6
作者：
Koh, Isaac;Hong, Rid;Josephson, Lee
通讯作者：
Josephson, Lee
影响因子：
4.7
作者：
Kim, Grace Y.;Josephson, Lee;Cima, Michael J.
通讯作者：
Cima, Michael J.
影响因子：
15
作者：
Perez, JM;Simeone, FJ;Weissleder, R
通讯作者：
Weissleder, R
DOI：
10.1073/pnas.0607991103
发表时间：
2006-10-31
影响因子：
11.1
作者：
Baudry, J.;Rouzeau, C.;Bibette, J.
通讯作者：
Bibette, J.