Expression of D-type cyclins in colon cancer and in cell lines from colon carcinomas.
Expression of D-type cyclins in colon cancer and in cell lines from colon carcinomas.
复制标题
DOI：
10.1038/sj.bjc.6602709
复制
发表时间：
2005-08-08
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
--
作者：
细胞周期蛋白D_1、D_2和D_3在细胞增殖和分化中发挥重要作用。尽管它们的异常表达与许多组织的癌症发生和发展有关，但细胞周期蛋白D2和D3在结肠癌中的表达尚未确定。在这项研究中，我们用免疫印迹法检测了57例患者肿瘤及癌旁正常结肠组织中细胞周期蛋白D1、D2和D3的表达。此外，我们还研究了结肠癌HT29和LoVo39细胞系中D-型细胞周期蛋白的表达与诱导增殖和分化的关系。在这两种细胞系中，三种D型细胞周期蛋白的表达都是诱导增殖的结果，而细胞周期蛋白D3的表达是诱导分化的结果。在结肠肿瘤中，Cyclin D1过表达占44%，Cyclin D2过表达占53%，Cyclin D3过表达占35%。我们还发现，在16%的病例中，与邻近的正常组织相比，肿瘤中细胞周期蛋白D3的表达降低。对D型细胞周期蛋白过度表达与肿瘤TNM分期关系的研究表明，细胞周期蛋白D1和/或D2的过度表达与结肠癌的发生有关，而与细胞周期蛋白D3无关，细胞周期蛋白D2的过度表达可能与肿瘤的较高TNM分期有关。
Cyclins D1, D2 and D3 play important roles in cell proliferation and differentiation. Although their abnormal expression has been linked to cancer development and progression in a number of tissues, the expression of cyclin D2 and D3 proteins in colon cancer has not yet been characterised. In this study, we examined cyclin D1, D2 and D3 protein expression by Western blot analysis in tumour and adjacent normal colon tissues of 57 patients. In addition, we examined D-type cyclins protein expression in HT29 and LoVo39 cell lines from colon carcinomas, as a function of induced proliferation and differentiation. In both cell lines, the expression of the three D-type cyclins increased as a result of induced proliferation, whereas the expression of cyclin D3 increased as a result of induced differentiation. In colon tumours, cyclin D1 was overexpressed in 44%, cyclin D2 was overexpressed in 53% and cyclin D3 was overexpressed in 35% of the cases. We also found that in 16% of the cases, cyclin D3 protein expression was reduced in the tumour, as compared to the adjacent normal tissue. Examination of D-type cyclin protein overexpression in relation to the TNM stage of the tumours revealed that overexpression of cyclins D1 and/or D2, but not cyclin D3, is linked to colon carcinogenesis and that overexpression of cyclin D2 may be related to a higher TNM stage of the tumour.
登录
查看更多内容
DOI：
10.1073/pnas.90.24.11513
发表时间：
1993-12-15
影响因子：
11.1
作者：
KATO, JY;SHERR, CJ
通讯作者：
SHERR, CJ
影响因子：
2.2
作者：
LUKAS, J;BARTEK, J;STRAUSS, M
通讯作者：
STRAUSS, M
影响因子：
6.2
作者：
SONGSTER, CL;BARROWS, GH;JARRETT, DD
通讯作者：
JARRETT, DD
影响因子：
8
作者：
Bartkova, J;Lukas, J;Bartek, J
通讯作者：
Bartek, J
影响因子：
64.5
作者：
MATSUSHIME, H;EWEN, ME;SHERR, CJ
通讯作者：
SHERR, CJ