Elucidation on Predominant Pathways Involved in the Differentiation and Mineralization of Odontoblast-Like Cells by Selective Blockade of Mitogen-Activated Protein Kinases.
Elucidation on Predominant Pathways Involved in the Differentiation and Mineralization of Odontoblast-Like Cells by Selective Blockade of Mitogen-Activated Protein Kinases.
复制标题
DOI：
10.1155/2018/2370438
复制
发表时间：
2018
影响因子：
--
通讯作者：
Saito T
中科院分区：
文献类型：
--
作者：
Tang J;Saito T
目的：分析丝裂原活化蛋白激酶(MAPK)抑制剂SB202190(p38抑制剂)、SP600125(JNK抑制剂)和PD98059(ERK抑制剂)在地塞米松刺激的MDPC-23细胞分化和矿化中的作用。实验分为5组，对照组(不加地塞米松和抑制剂的细胞)、地塞米松(地塞米松处理但不加抑制剂的细胞)、地塞米松+SB202190、地塞米松+SP600125、地塞米松+PD98059。碱性磷酸酶(ALP)活性测定和实时荧光定量RT-PCR检测细胞分化程度。茜素红染色观察细胞矿化情况。茜素红染色显示，暴露于SB202190(20 μM)可显著减少地塞米松处理细胞的矿物质沉积。与SB202190(20 μM)相比，SP600125(20 μM)处理也减弱了矿化作用，但程度较小。同样，SB202190(20 μM)可完全阻断地塞米松刺激培养6天的细胞碱性磷酸酶活力，而对SP600125(20 μM)和PD98059(20 μM)的碱性磷酸酶活力无明显影响。SB202190可完全抑制Dex刺激的细胞中骨唾液酸蛋白(BSP)、碱性磷酸酶(ALP)和骨桥蛋白(OPN)的上调。阻断p38-MAPK信号通路可显著抑制ALP活性、矿化和成骨标志物的下调。提示p38信号通路在MDPC-23细胞分化和矿化的调控中起关键作用。
To analyze the effect of three mitogen-activated protein kinase (MAPK) inhibitors, namely, SB202190 (p38 inhibitor), SP600125 (JNK inhibitor), and PD98059 (ERK inhibitor) in Dex-stimulated MDPC-23 cell differentiation and mineralization. Experiment was divided into five groups, control (cells without Dex and inhibitors treatment), Dex (cells with Dex treatment but without inhibitors), Dex + SB202190, Dex + SP600125, and Dex + PD98059. Cell differentiation was assessed by alkaline phosphatase (ALP) activity assay and real time RT-PCR. Cell mineralization was investigated by alizarin red staining. Exposure to SB202190 (20 μM) significantly decreased the mineral deposition in Dex-treated cells as demonstrated by alizarin red staining. Treatment of SP600125 (20 μM) attenuated the mineralization as well, albeit at a lower degree as compared to SB202190 (20 μM). Similarly, SB202190 (20 μM) completely abrogated the ALP activity stimulated by Dex at six days in culture, while no changes were observed with regard to ALP activity in SP600125 (20 μM) and PD98059 (20 μM) treated cells. The upregulation of bone sialoprotein (BSP), ALP, and osteopontin (OPN) in Dex challenged cells was completely inhibited by SB202190. Blockade of p38-MAPK signaling pathway resulted in significant inhibition of ALP activity, mineralization, and downregulation of osteogenic markers. The data implicated that p38 signaling pathway plays a critical role in the regulation of MDPC-23 cells differentiation and mineralization.
登录
查看更多内容
影响因子：
7.5
作者：
Langenbach F;Handschel J
通讯作者：
Handschel J
氢氧化钙通过丝裂原激活蛋白激酶途径诱导人牙髓干细胞的增殖、迁移、成骨分化和矿化
影响因子：
4.2
作者：
Chen, Luoping;Zheng, Lisha;Fan, Yubo
通讯作者：
Fan, Yubo
影响因子：
2.9
作者：
Qin, Wei;Liu, Pengcheng;Lin, Zhengmei
通讯作者：
Lin, Zhengmei
影响因子：
168.9
作者：
Elder, Charlotte Jane;Bishop, Nicholas J.
通讯作者：
Bishop, Nicholas J.
DOI：
10.1155/2012/308943
发表时间：
2012
期刊：
Journal of signal transduction
影响因子：
--
作者：
Li Q;Valerio MS;Kirkwood KL
通讯作者：
Kirkwood KL