Hierarchical change in antioxidant enzyme gene expression and activity in acute cardiac rejection: role of inducible nitric oxide synthase.
Hierarchical change in antioxidant enzyme gene expression and activity in acute cardiac rejection: role of inducible nitric oxide synthase.
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急性心脏排斥反应中抗氧化酶基因表达和活性的层次变化：诱导型一氧化氮合酶的作用。
DOI：
10.1007/s11010-005-3639-2
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发表时间：
2005
影响因子：
4.3
通讯作者：
Pieper,GalenM
中科院分区：
文献类型：
--
作者：
Nilakantan,Vani;Zhou,Xianghua;Hilton,Gail;Roza,AllanM;Adams,MarkB;Johnson,ChristopherP;Pieper,GalenM
Reactive oxygen and nitrogen may mediate inflammation injury, but the status of the antioxidant defense system that might influence this process is unknown. In the present study, we examined the expression profile of the antioxidant enzymes, manganese superoxide dismutase (MnSOD), catalase and glutathione peroxidase (GPX) in acutely rejecting cardiac allografts and the potential role of inducible nitric oxide synthase (iNOS) in modulating antioxidant gene expression and activity. Donor hearts from Lewis (isograft) or Wistar-Furth (allograft) rats were transplanted into Lewis recipient rats. A subset of the allografts received l-N6-(1-imino-ethyl) lysine (l-NIL), a specific iNOS inhibitor, beginning the day of surgery until the day of harvesting. Catalase and glutathione peroxidase (GPX) protein levels were significantly decreased by postoperative day 4 (POD4) and postoperative day 5 (POD5), respectively, in allografts compared to isografts. While CuZn superoxide dismutase (CuZn SOD) levels were unchanged, there was a 50% decrease in MnSOD protein in allografts at postoperative day 6 (POD6). The sequential loss in antioxidant protein levels was not due to transcriptional regulation since there was no change in RNA levels for any of the genes tested. l-NIL did not alter catalase protein; however, the loss of MnSOD protein at POD6 was prevented by l-NIL. Consistent with a decrease in antioxidant protein levels, there was a sequential loss in enzyme activity for MnSOD, catalase and GPX. l-NIL however, restored MnSOD and GPX activities but not catalase activity. Treatment with CsA restored both protein and enzyme activities of GPX and MnSOD but not catalase. These results indicate that the loss in MnSOD and GPX protein and activity in allografts occurs via an iNOS-dependent mechanism whereas the decrease in catalase appears to be iNOS-independent. This suggests a differential role for iNOS in regulating post-translational modification of individual antioxidant enzymes in acute cardiac transplantation. (Mol Cell Biochem270: 39–47, 2005)
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影响因子：
4.8
作者：
A. Midgley
通讯作者：
A. Midgley
DOI：
10.1016/0010-4809(86)90014-5
发表时间：
1986-04-01
期刊：
COMPUTERS AND BIOMEDICAL RESEARCH
影响因子：
--
作者：
OERTER, KE;GUARDABASSO, V;RODBARD, D
通讯作者：
RODBARD, D
影响因子：
15.9
作者：
SANTEN, RJ;BARDIN, CW
通讯作者：
BARDIN, CW
青春期前后女孩和男孩血清促性腺激素的间歇性波动。
DOI：
10.1210/jcem-45-2-307
发表时间：
1977
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
R. Penny;N. O. Olambiwonnu;S. Frasier
通讯作者：
S. Frasier
烧伤男性的垂体-睾丸轴功能障碍。
DOI：
10.1210/jcem-60-4-658
发表时间：
1985
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
Vogel,AV;Peake,GT;Rada,RT
通讯作者：
Rada,RT