Mouse spermatogenic stem cells continually interconvert between equipotent singly isolated and syncytial states.
Mouse spermatogenic stem cells continually interconvert between equipotent singly isolated and syncytial states.
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DOI：
10.1016/j.stem.2014.01.019
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发表时间：
2014-05-01
期刊：
影响因子：
23.9
通讯作者：
Yoshida, Shosei
中科院分区：
文献类型：
--
作者：
Hara, Kenshiro;Nakagawa, Toshinori;Enomoto, Hideki;Suzuki, Mikiko;Yamamoto, Masayuki;Simons, Benjamin D.;Yoshida, Shosei
小鼠生精干细胞的特性和行为一直是人们关注的焦点。在流行的“As模型”中，干细胞功能仅限于单个分离的精原细胞。通过检查体内GFRα1+干细胞的单细胞动力学，我们评估了另一种假设，即通过分裂，合胞精原细胞也有助于干细胞功能的稳态。我们使用活体成像和脉冲标记来定量确定单个GFRα1+细胞的命运，并发现在稳态精子发生期间，整个GFRα1+群体包括单个干细胞库，其中细胞在As和合胞体状态之间不断相互转换。一个最小的生物物理模型，仅依赖于不完全的细胞分裂和合胞体碎裂的速率，精确地预测了稳态和损伤后再生期间GFRα1+细胞的随机命运。因此，我们的研究结果定义了一个替代的和动态的模型，在小鼠睾丸生精干细胞的功能。GFRα1+精原细胞在体内平衡期间构成单个干细胞池GFRα1+精原细胞在单个分离状态和合胞状态之间相互转换不完全分裂和合胞碎片化的速率控制干细胞动力学GFRα1+精原细胞的运动对于干细胞群体不对称性至关重要Hara et al.研究生精干细胞动力学，并证明在稳态精子发生过程中，精原细胞可以在功能等同的单个分离和合胞体干细胞状态之间相互转化。
The identity and behavior of mouse spermatogenic stem cells have been a long-standing focus of interest. In the prevailing “As model,” stem cell function is restricted to singly isolated (As) spermatogonia. By examining single-cell dynamics of GFRα1+ stem cells in vivo, we evaluate an alternative hypothesis that, through fragmentation, syncytial spermatogonia also contribute to stem cell function in homeostasis. We use live imaging and pulse labeling to quantitatively determine the fates of individual GFRα1+ cells and find that, during steady-state spermatogenesis, the entire GFRα1+ population comprises a single stem cell pool, in which cells continually interconvert between As and syncytial states. A minimal biophysical model, relying only on the rates of incomplete cell division and syncytial fragmentation, precisely predicts the stochastic fates of GFRα1+ cells during steady state and postinsult regeneration. Thus, our results define an alternative and dynamic model for spermatogenic stem cell function in the mouse testis. GFRα1+ spermatogonia comprise a single stem cell pool during homeostasis GFRα1+ spermatogonia interconvert between singly isolated and syncytial states Rates of incomplete division and syncytial fragmentation govern stem cell dynamics Movement of GFRα1+ spermatogonia is essential for stem cell population asymmetry Hara et al. examine spermatogenic stem cell dynamics and demonstrate that spermatogonia can interconvert between functionally equivalent singly isolated and syncytial stem cell states during steady-state spermatogenesis.
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