Brain-derived neurotrophic factor and its receptor in the human and the sand rat intervertebral disc.
Brain-derived neurotrophic factor and its receptor in the human and the sand rat intervertebral disc.
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DOI：
10.1186/ar2456
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发表时间：
2008
影响因子：
4.9
通讯作者：
Hanley EN Jr
中科院分区：
文献类型：
--
作者：
Gruber HE;Ingram JA;Hoelscher G;Zinchenko N;Norton HJ;Hanley EN Jr
脑源性神经营养因子（BDNF）首次在椎间盘（IVD）中被鉴定，当时在渗透压增加的条件下培养的髓核切片中观察到其分子上调。目前已知BDNF参与许多生物学功能，包括调节感觉神经元的分化/存活、调节伤害感受功能和中枢疼痛调节以及调节炎性疼痛超敏性。此外，最近的研究表明，BDNF可以诱导内皮细胞的募集和血管结构的形成。本研究的目的是用免疫细胞化学方法检测BDNF及其受体（BDNF-原肌氨酸受体激酶B）在人椎间盘中的分布，并检测BDNF及其受体在培养的人纤维环细胞中的基因表达。我们研究了BDNF及其受体在人纤维环中的免疫组织化学定位，定量了BDNF免疫定位阳性的外环和内环细胞以及核细胞的百分比，并使用微阵列分析研究了BDNF及其受体的基因表达。BDNF定位阳性细胞百分比（平均值±平均值的标准误差）在外环（32.3 ± 2.7%，n = 22）中显著高于内环（8.1 ± 1.5%，n = 6）或核（10.4 ± 2.8%，n = 3）（P < 0.0001）。BDNF受体免疫定位显示出类似的模式，BDNF，但没有定量评估。BDNF基因表达水平与椎间盘退变程度呈显著正相关（P = 0.011）。这些发现提供了BDNF及其受体在人IVD中的翻译水平的存在的数据，以及培养的人纤维环细胞的BDNF及其受体的表达。我们的研究结果指出，需要进一步的研究，以确定BDNF在人类IVD的作用，并调查控制BDNF及其受体表达的椎间盘内的监管事件。
Brain-derived neurotrophic factor (BDNF) was first identified in the intervertebral disc (IVD) when its molecular upregulation was observed in sections of nucleus pulposus cultured under conditions of increased osmolarity. BDNF is now known to be involved in a number of biologic functions, including regulation of differentiation/survival of sensory neurons, regulation of nociceptive function and central pain modulation, and modulation of inflammatory pain hypersensitivity. In addition, more recent investigations show that BDNF can induce the recruitment of endothelial cells and the formation of vascular structures. The objectives of the present study were to use immunocytochemistry to determine the distribution of BDNF and its receptor (BDNF-tropomyosine receptor kinase B) in the human IVD, and to test for gene expression of BDNF and its receptor in cultured human annulus fibrosus cells. We studied immunohistochemical localization of BDNF and its receptor in the human annulus, quantified the percentage of outer annulus and inner annulus cells and nucleus cells positive for BDNF immunolocalization, and studied the gene expression of BDNF and its receptor using microarray analysis. The percentage (mean ± standard error of the mean) of cells positive for BDNF localization was significantly greater in the outer annulus (32.3 ± 2.7%, n = 22) compared with either the inner annulus (8.1 ± 1.5%, n = 6) or the nucleus (10.4 ± 2.8%, n = 3) (P < 0.0001). BDNF-receptor immunolocalization showed a pattern similar to that of BDNF, but was not quantitatively assessed. BDNF gene expression levels from cultured annulus cells showed a significant positive correlation with increasing levels of IVD degeneration (P = 0.011). These findings provide data on the presence of BDNF and its receptor in the human IVD at the translational level, and on the expression of BDNF and its receptor by cultured human annulus cells. Our findings point to the need for further studies to define the role of BDNF in the human IVD and to investigate regulatory events within the disc that control the expression of BDNF and its receptor.
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影响因子：
4.9
作者：
Le Maitre, Christine Lyn;Hoyland, Judith Alison;Freemont, Anthony J
通讯作者：
Freemont, Anthony J
影响因子：
3
作者：
THOMPSON, JP;PEARCE, RH;BISHOP, PB
通讯作者：
BISHOP, PB
影响因子：
5.3
作者：
Guo, W;Robbins, MT;Ren, K
通讯作者：
Ren, K
影响因子：
3
作者：
Gruber, HE;Johnson, T;Hanley, EN
通讯作者：
Hanley, EN
影响因子：
2.1
作者：
Pagnotta, A;Tos, P;Battiston, B
通讯作者：
Battiston, B