Proteomic analysis identifies proteins that continue to grow hepatic stem-like cells without differentiation.
Proteomic analysis identifies proteins that continue to grow hepatic stem-like cells without differentiation.
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DOI：
10.1007/s10616-008-9122-7
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发表时间：
2008-06
期刊：
影响因子：
2.2
通讯作者：
Kojima, Itaru
中科院分区：
文献类型：
--
作者：
Saheki, Toshihiko;Ito, Hitomi;Sekiguchi, Akihiro;Nishina, Atsuyoshi;Sugiyama, Toshihiro;Izumi, Takashi;Kojima, Itaru
为了了解肝干细胞样（HSL）细胞剧烈增殖活性的分子机制，我们进行了双向电泳，以确定蛋白质在快速生长的未分化的HSL细胞比丁酸钠处理的分化的HSL细胞在统计学上更丰富。基质辅助激光解吸/电离飞行时间质谱和Mascot搜索鉴定了6种蛋白质，包括prohibitin，vimentin，ezrin，annexin A3，酸性核糖体磷蛋白P0和Grp 75。抑制素和波形蛋白控制丝裂原活化蛋白（MAP）激酶途径。Ezrin被各种蛋白酪氨酸激酶磷酸化，并调节细胞骨架和膜蛋白之间的相互作用。膜联蛋白A3在DNA合成中发挥作用。酸性核糖体磷蛋白P0和Grp 75在蛋白质合成中起作用。这些结果表明，MAP激酶级联相关蛋白在HSL细胞的持续增殖而不分化中具有一定的作用。
To understand the molecular mechanism underlying vigorous proliferative activity of hepatic stem-like (HSL) cells, we performed two-dimensional electrophoresis to identify the proteins statistically more abundant in rapidly growing undifferentiated HSL cells than in sodium butyrate-treated differentiated HSL cells. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry and Mascot search identified 6 proteins including prohibitin, vimentin, ezrin, annexin A3, acidic ribosomal phosphoprotein P0 and Grp75. Prohibitin and vimentin control the mitogen-activated protein (MAP) kinase pathway. Ezrin is phosphorylated by various protein-tyrosine kinases and modulates interactions between cytoskeletal and membrane proteins. Annexin A3 has a role in DNA synthesis. Acidic ribosomal phosphoprotein P0 and Grp75 play in protein synthesis. These results suggest that the proteins related to the MAP kinase cascade had some role in continuous proliferation of HSL cells without differentiation.
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DOI：
10.1111/j.1582-4934.2006.tb00404.x
发表时间：
2006-04
影响因子：
5.3
作者：
Mishra S;Murphy LC;Murphy LJ
通讯作者：
Murphy LJ
DOI：
10.1016/s0006-291x(02)00406-0
发表时间：
2002-05-24
影响因子：
3.1
作者：
Nagai, H;Terada, K;Sugiyama, T
通讯作者：
Sugiyama, T
DOI：
10.1016/s0006-291x(02)02911-x
发表时间：
2003-01-17
影响因子：
3.1
作者：
Niimi, S;Oshizawa, T;Hayakawa, T
通讯作者：
Hayakawa, T
影响因子：
4.3
作者：
Rajalingam, K;Rudel, T
通讯作者：
Rudel, T
影响因子：
3.3
作者：
Srivastava, J;Elliott, BE;Arpin, M
通讯作者：
Arpin, M