Spine-to-Dendrite Calcium Modeling Discloses Relevance for Precise Positioning of Ryanodine Receptor-Containing Spine Endoplasmic Reticulum.
Spine-to-Dendrite Calcium Modeling Discloses Relevance for Precise Positioning of Ryanodine Receptor-Containing Spine Endoplasmic Reticulum.
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DOI：
10.1038/s41598-018-33343-9
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发表时间：
2018-10-23
影响因子：
4.6
通讯作者：
Queisser G
中科院分区：
文献类型：
--
作者：
Breit M;Kessler M;Stepniewski M;Vlachos A;Queisser G
内质网（ER）形成一个复杂的内膜网络，可以进入神经元的细胞室，包括树突棘。最近的研究表明，脊柱内质网是一个进入和离开脊柱的动态结构。虽然有证据表明内质网Ca2+释放参与突触可塑性，但脊柱内质网形态的作用仍不清楚。结合新的3D脊柱生成器和3D Ca2+建模，我们解决了内质网定位与脊柱到树突Ca2+信号的相关性。我们的模拟计算了质膜和内质网上的Ca2+交换，表明脊柱内质网需要以不同的形态构象存在，以克服脊柱和树突轴之间的屏障。我们证明携带ryr的脊柱ER以位置依赖的方式促进脊柱到树突的Ca2+信号。我们的模拟表明，携带ryr的内质网可以启动延时Ca2+混响，这取决于脊柱内质网的精确位置。在脊柱生长时，内质网的结构重组恢复了脊柱与树突Ca2+的通信，同时维持了脊柱头部Ca2+的稳态。我们的工作强调了含有ryr的脊柱内质酶的精确定位与调节脊柱Ca2+信号的强度和时间的相关性，这可能在调节脊柱与树突Ca2+通信和体内平衡中发挥重要作用。
The endoplasmic reticulum (ER) forms a complex endomembrane network that reaches into the cellular compartments of a neuron, including dendritic spines. Recent work discloses that the spine ER is a dynamic structure that enters and leaves spines. While evidence exists that ER Ca2+ release is involved in synaptic plasticity, the role of spine ER morphology remains unknown. Combining a new 3D spine generator with 3D Ca2+ modeling, we addressed the relevance of ER positioning on spine-to-dendrite Ca2+ signaling. Our simulations, which account for Ca2+ exchange on the plasma membrane and ER, show that spine ER needs to be present in distinct morphological conformations in order to overcome a barrier between the spine and dendritic shaft. We demonstrate that RyR-carrying spine ER promotes spine-to-dendrite Ca2+ signals in a position-dependent manner. Our simulations indicate that RyR-carrying ER can initiate time-delayed Ca2+ reverberation, depending on the precise position of the spine ER. Upon spine growth, structural reorganization of the ER restores spine-to-dendrite Ca2+ communication, while maintaining aspects of Ca2+ homeostasis in the spine head. Our work emphasizes the relevance of precise positioning of RyR-containing spine ER in regulating the strength and timing of spine Ca2+ signaling, which could play an important role in tuning spine-to-dendrite Ca2+ communication and homeostasis.
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影响因子：
16.2
作者：
Bosch M;Castro J;Saneyoshi T;Matsuno H;Sur M;Hayashi Y
通讯作者：
Hayashi Y
DOI：
10.1073/pnas.89.20.9895
发表时间：
1992-10-15
影响因子：
11.1
作者：
DEYOUNG, GW;KEIZER, J
通讯作者：
KEIZER, J
影响因子：
4.8
作者：
Elwess, NL;Filoteo, AG;Penniston, JT
通讯作者：
Penniston, JT
影响因子：
2.4
作者：
CHIU, VCK;HAYNES, DH
通讯作者：
HAYNES, DH
影响因子：
16.2
作者：
Cartailler J;Kwon T;Yuste R;Holcman D
通讯作者：
Holcman D