Geographic distribution and genetic characterization of Lassa virus in sub-Saharan Mali.
Geographic distribution and genetic characterization of Lassa virus in sub-Saharan Mali.
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DOI：
10.1371/journal.pntd.0002582
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发表时间：
2013
影响因子：
3.8
通讯作者：
Feldmann H
中科院分区：
文献类型：
--
作者：
Safronetz D;Sogoba N;Lopez JE;Maiga O;Dahlstrom E;Zivcec M;Feldmann F;Haddock E;Fischer RJ;Anderson JM;Munster VJ;Branco L;Garry R;Porcella SF;Schwan TG;Feldmann H
拉沙热是一种急性病毒性疾病，其特征是多器官衰竭和出血表现。拉沙热最常见于尼日利亚、塞拉利昂、利比里亚和几内亚，但在包括马里在内的其他西非国家也有零星病例。拉沙热的病原体是拉沙病毒（LASV），这是一种沙粒病毒，在自然界中存在，经常通过纳他乳鼠传播给人类。本研究的目的是更好地确定撒哈拉以南马里地区感染LASV的啮齿动物的地理分布。在马里各地对小型哺乳动物进行了活捕，以确定潜在的人畜共患病原体。采用了血清学和分子检测，确定感染LASV的啮齿动物仅在马里南部靠近科特迪瓦边界的地区发现。总体而言，19.4%的Mastomys natalensis采样在该地区有LASV感染的证据，个别村庄的患病率从0到52%不等。全长基因组序列测定使用高通量测序方法产生的LASV分离物的组织样本中收集的啮齿动物在四个村庄，并确认了系统发育聚类的马里LASV株AV。在马里南部的村庄，人感染LASV的风险最大。在发热个体的鉴别诊断中应考虑拉沙热，需要建立适当的诊断技术以确定这些地区的感染和疾病发生率。拉沙热是西非一种与出血性表现和多器官衰竭相关的急性感染。拉沙热的病原体是拉沙病毒（LASV），一种啮齿动物传播的沙粒病毒，在自然界中存在并通过多乳鼠（Mastomys natalensis）传播给人类。尽管啮齿类动物的储库无处不在，LASV感染的动物最常见的记录在尼日利亚，塞拉利昂，几内亚和利比里亚。这四个国家代表了拉沙热的历史流行地区，尽管越来越多的证据表明，包括马里在内的其他西非国家也发生了零星病例。为了更好地确定LASV感染的啮齿动物在马里的地理分布，我们测试了从全国27个地点收集的小动物样本。虽然M.虽然大多数村庄的啮齿类动物主要是纳塔尔人，但LASV感染的证据仅在马里南部发现，那里总共有近20%的啮齿类动物呈阳性。测定了5个分离株的全基因组序列，证实马里的LASV与AV株密切相关。我们的结论是，在马里南部的村庄，人类接触LASV的风险和拉沙热应考虑在急性病，发热患者的鉴别诊断。
Lassa fever is an acute viral illness characterized by multi-organ failure and hemorrhagic manifestations. Lassa fever is most frequently diagnosed in Nigeria, Sierra Leone, Liberia, and Guinea, although sporadic cases have been recorded in other West African countries, including Mali. The etiological agent of Lassa fever is Lassa virus (LASV), an Arenavirus which is maintained in nature and frequently transmitted to humans by Mastomys natalensis. The purpose of this study was to better define the geographic distribution of LASV-infected rodents in sub-Saharan Mali. Small mammals were live-trapped at various locations across Mali for the purpose of identifying potential zoonotic pathogens. Serological and molecular assays were employed and determined LASV infected rodents were exclusively found in the southern Mali near the border of Côte d'Ivoire. Overall, 19.4% of Mastomys natalensis sampled in this region had evidence of LASV infection, with prevalence rates for individual villages ranging from 0 to 52%. Full-length genomic sequences were determined using high throughput sequencing methodologies for LASV isolates generated from tissue samples of rodents collected in four villages and confirmed the phylogenetic clustering of Malian LASV with strain AV. The risk of human infections with LASV is greatest in villages in southern Mali. Lassa fever should be considered in the differential diagnosis for febrile individuals and appropriate diagnostic techniques need to be established to determine the incidence of infection and disease in these regions. Lassa fever is an acute infection associated with hemorrhagic manifestations and multi-organ failure in West Africa. The etiological agent of Lassa fever is Lassa virus (LASV), a rodent-borne arenavirus, which is maintained in nature and transmitted to humans by the multimammate rat, Mastomys natalensis. Despite the ubiquitous nature of the rodent reservoir, LASV-infected animals are most commonly documented in Nigeria, Sierra Leone, Guinea and Liberia. These four countries represent the historic endemic region for Lassa fever, although there is increasing evidence of sporadic cases occurring in other West African nations including Mali. To better define the geographic distribution of LASV-infected rodents in Mali, we tested samples from small animals collected at 27 sites across the country. Although M. natalensis was the predominant rodent species in the majority of villages, evidence of LASV infection was exclusively found in southern Mali, where overall nearly 20% of rodents were positive. The full genomic sequence was determined for five isolates and confirmed LASV in Mali is closely related to strain AV. We conclude that there is a risk of human exposure to LASV in villages in southern Mali and Lassa fever should be considered in the differential diagnosis for acutely ill, febrile patients.
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影响因子：
64.8
作者：
Keesing F;Belden LK;Daszak P;Dobson A;Harvell CD;Holt RD;Hudson P;Jolles A;Jones KE;Mitchell CE;Myers SS;Bogich T;Ostfeld RS
通讯作者：
Ostfeld RS
DOI：
10.4269/ajtmh.1970.19.670
发表时间：
1970-01-01
影响因子：
3.3
作者：
FRAME, JD;BALDWIN, JM;TROUP, JM
通讯作者：
TROUP, JM
影响因子：
7.6
作者：
Charrel, RN;de Lamballerie, X
通讯作者：
de Lamballerie, X
影响因子：
--
作者：
FISHERHOCH, SP;TOMORI, O;MCCORMICK, JB
通讯作者：
MCCORMICK, JB
影响因子：
3.8
作者：
Fichet-Calvet, Elisabeth;Rogers, David John
通讯作者：
Rogers, David John