Alteration of Bacterial Communities in Anterior Nares and Skin Sites of Patients Undergoing Arthroplasty Surgery: Analysis by 16S rRNA and Staphylococcal-Specific tuf Gene Sequencing.
Alteration of Bacterial Communities in Anterior Nares and Skin Sites of Patients Undergoing Arthroplasty Surgery: Analysis by 16S rRNA and Staphylococcal-Specific tuf Gene Sequencing.
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DOI：
10.3390/microorganisms8121977
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发表时间：
2020-12-12
期刊：
影响因子：
4.5
通讯作者：
Andersen PS
中科院分区：
文献类型：
--
作者：
Iversen S;Johannesen TB;Ingham AC;Edslev SM;Tevell S;Månsson E;Nilsdotter-Augustinsson Å;Söderquist B;Stegger M;Andersen PS
目的是研究髋关节或膝关节置换术患者细菌群落的变化，以评估葡萄糖酸洗必泰肥皂去克隆和全身抗生素预防的影响。通过擦拭前鼻孔、腹股沟皮肤部位和计划手术部位，瑞典多中心、前瞻性样本采集自择期关节置换患者(n=83)，通过16S rRNA(V3-V4)基因测序和互补靶向TUF基因测序方法进行分析，以全面描述葡萄球菌群落的变化。关节置换术后腹股沟细菌(p=0.04)和葡萄球菌(p=0.03)群落的α多样性显著降低，棒状杆菌(p=0.001)相对丰度和人葡萄球菌(p=0.01)相对丰度显著降低。在NAARES中，与其他葡萄球菌相比，人葡萄球菌(p=0.02)、溶血葡萄球菌(p=0.02)和巴氏葡萄球菌(p=0.003)的感染率显著下降。金黄色葡萄球菌在前鼻孔定植了35%，在关节成形术后定植了26%。在所有采样点中，表皮葡萄球菌是最多的葡萄球菌。关节置换术后无细菌属或葡萄球菌属明显增加。应用有针对性的TUF基因测序方法提供了辅助的葡萄球菌群落特征，并允许直接从低生物量临床样本中确定物种水平的特征。
The aim was to study alterations of bacterial communities in patients undergoing hip or knee arthroplasty to assess the impact of chlorhexidine gluconate soap decolonisation and systemic antibiotic prophylaxis. A Swedish multicentre, prospective collection of samples obtained from elective arthroplasty patients (n = 83) by swabbing anterior nares, skin sites in the groin and the site of planned surgery, before and after arthroplasty surgery, was analysed by 16S rRNA (V3-V4) gene sequencing and a complementary targeted tuf gene sequencing approach to comprehensively characterise alterations in staphylococcal communities. Significant reductions in alpha diversity was detected for both bacterial (p = 0.04) and staphylococcal (p = 0.03) groin communities after arthroplasty surgery with significant reductions in relative Corynebacterium (p = 0.001) abundance and Staphylococcus hominis (p = 0.01) relative staphylococcal abundance. In nares, significant reductions occurred for Staphylococcus hominis (p = 0.02), Staphylococcus haemolyticus (p = 0.02), and Staphylococcus pasteuri (p = 0.003) relative to other staphylococci. Staphylococcus aureus colonised 35% of anterior nares before and 26% after arthroplasty surgery. Staphylococcus epidermidis was the most abundant staphylococcal species at all sampling sites. No bacterial genus or staphylococcal species increased significantly after arthroplasty surgery. Application of a targeted tuf gene sequencing approach provided auxiliary staphylococcal community profiles and allowed species-level characterisation directly from low biomass clinical samples.
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影响因子：
4.4
作者：
MacConaill LE;Burns RT;Nag A;Coleman HA;Slevin MK;Giorda K;Light M;Lai K;Jarosz M;McNeill MS;Ducar MD;Meyerson M;Thorner AR
通讯作者：
Thorner AR
影响因子：
15.5
作者：
Davis, Nicole M;Proctor, Diana M;Callahan, Benjamin J
通讯作者：
Callahan, Benjamin J
影响因子：
1.7
作者：
Harris, Llinos G.;El-Bouri, Khalid;Mack, Dietrich
通讯作者：
Mack, Dietrich
影响因子：
28.3
作者：
Lee JYH;Monk IR;Gonçalves da Silva A;Seemann T;Chua KYL;Kearns A;Hill R;Woodford N;Bartels MD;Strommenger B;Laurent F;Dodémont M;Deplano A;Patel R;Larsen AR;Korman TM;Stinear TP;Howden BP
通讯作者：
Howden BP
影响因子：
64.5
作者：
Oh J;Byrd AL;Park M;NISC Comparative Sequencing Program;Kong HH;Segre JA
通讯作者：
Segre JA