Global transcriptional analysis of Burkholderia pseudomallei high and low biofilm producers reveals insights into biofilm production and virulence.
Global transcriptional analysis of Burkholderia pseudomallei high and low biofilm producers reveals insights into biofilm production and virulence.
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DOI：
10.1186/s12864-015-1692-0
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发表时间：
2015-06-20
期刊：
影响因子：
4.4
通讯作者：
Nathan S
中科院分区：
文献类型：
--
作者：
Chin CY;Hara Y;Ghazali AK;Yap SJ;Kong C;Wong YC;Rozali N;Koh SF;Hoh CC;Puthucheary SD;Nathan S
慢性细菌感染的发生是由于感染病原体能够在生物膜内生存，从而逃避抗生素和免疫防御系统的有害影响。假性伯克霍尔德菌是一种革兰氏阴性兼性病原体，其独特的能力是在吞噬细胞和非吞噬细胞中存活，在体内持续多年，随后导致复发和慢性疾病的发展。这种持续存在的能力归因于病原体形成生物膜的能力。然而，假芽孢杆菌生物膜发育的生物学基础仍未得到解决。我们利用rna测序来鉴定导致假芽孢杆菌生物膜表型的基因。对高、低生物膜产生菌的转录组分析鉴定出563个差异调控基因，表明在生物膜形成过程中，约9.5%的假假芽孢杆菌基因表达发生了改变。参与表面相关运动、表面组成和细胞壁生物发生的基因被过度表达，并可能在生物膜的初始附着中发挥作用。双组分信号转导系统和反硝化酶途径相关基因的上调表明假假芽孢杆菌高生物膜产生菌能够感知周围环境条件并调节细胞外聚合物基质的产生，这是微生物生物膜形成的标志。这里描述的转录组谱提供了第一个全面的基因视图，有助于假芽孢杆菌的生物膜表型。本文的在线版本（doi:10.1186/s12864-015-1692-0）包含补充材料，可供授权用户使用。
Chronic bacterial infections occur as a result of the infecting pathogen’s ability to live within a biofilm, hence escaping the detrimental effects of antibiotics and the immune defense system. Burkholderia pseudomallei, a gram-negative facultative pathogen, is distinctive in its ability to survive within phagocytic and non-phagocytic cells, to persist in vivo for many years and subsequently leading to relapse as well as the development of chronic disease. The capacity to persist has been attributed to the pathogen’s ability to form biofilm. However, the underlying biology of B. pseudomallei biofilm development remains unresolved. We utilised RNA-Sequencing to identify genes that contribute to B. pseudomallei biofilm phenotype. Transcriptome analysis of a high and low biofilm producer identified 563 differentially regulated genes, implying that expression of ~9.5 % of the total B. pseudomallei gene content was altered during biofilm formation. Genes involved in surface-associated motility, surface composition and cell wall biogenesis were over-expressed and probably play a role in the initial attachment of biofilms. Up-regulation of genes related to two component signal transduction systems and a denitrification enzyme pathway suggest that the B. pseudomallei high biofilm producer is able to sense the surrounding environmental conditions and regulate the production of extracellular polymeric substance matrix, a hallmark of microbial biofilm formation. The transcriptome profile described here provides the first comprehensive view of genes that contribute to the biofilm phenotype in B. pseudomallei. The online version of this article (doi:10.1186/s12864-015-1692-0) contains supplementary material, which is available to authorized users.
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影响因子：
6.4
作者：
Blanchette-Cain K;Hinojosa CA;Akula Suresh Babu R;Lizcano A;Gonzalez-Juarbe N;Munoz-Almagro C;Sanchez CJ;Bergman MA;Orihuela CJ
通讯作者：
Orihuela CJ
影响因子：
11
作者：
Boon, Calvin;Deng, Yinyue;Zhang, Lian-Hui
通讯作者：
Zhang, Lian-Hui
影响因子：
4.2
作者：
Brett, PJ;Deshazer, D;Woods, DE
通讯作者：
Woods, DE
影响因子：
3.1
作者：
Chen, Yahua;Wong, Jocelyn;Gan, Yunn-Hwen
通讯作者：
Gan, Yunn-Hwen
影响因子：
4.4
作者：
Chin CY;Monack DM;Nathan S
通讯作者：
Nathan S