In vitro circadian period is associated with circadian/sleep preference.
In vitro circadian period is associated with circadian/sleep preference.
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DOI：
10.1038/srep02074
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发表时间：
2013
影响因子：
4.6
通讯作者：
Mishima, Kazuo
中科院分区：
文献类型：
--
作者：
Hida, Akiko;Kitamura, Shingo;Ohsawa, Yosuke;Enomoto, Minori;Katayose, Yasuko;Motomura, Yuki;Moriguchi, Yoshiya;Nozaki, Kentaro;Watanabe, Makiko;Aritake, Sayaka;Higuchi, Shigekazu;Kato, Mie;Kamei, Yuichi;Yamazaki, Shin;Goto, Yu-ichi;Ikeda, Masaaki;Mishima, Kazuo
昼夜节律表型的评估对于理解与生物节律紊乱相关的疾病如昼夜节律睡眠障碍（CRSD）的病理生理学至关重要。我们测量了个体受试者成纤维细胞中的时钟基因表达，并观察了细胞中的昼夜节律（体外节律）。将体外节律的周期长度（体外周期）与在强迫性睡眠方案（体内周期）下测量的固有昼夜节律周期τ以及通过问卷调查、睡眠日志和体动记录仪评估的昼夜节律/睡眠参数进行比较。虽然在体外和体内期间之间没有观察到显著的相关性，但体外期间与时钟类型、习惯性睡眠时间和偏好睡眠时间相关。我们的数据表明，在体外期间与昼夜节律/睡眠偏好显着相关。研究结果表明，来自个体患者的成纤维细胞可用于体外筛选治疗药物，为CRSD患者提供个性化的治疗方案。
Evaluation of circadian phenotypes is crucial for understanding the pathophysiology of diseases associated with disturbed biological rhythms such as circadian rhythm sleep disorders (CRSDs). We measured clock gene expression in fibroblasts from individual subjects and observed circadian rhythms in the cells (in vitro rhythms). Period length of the in vitro rhythm (in vitro period) was compared with the intrinsic circadian period, τ, measured under a forced desynchrony protocol (in vivo period) and circadian/sleep parameters evaluated by questionnaires, sleep log, and actigraphy. Although no significant correlation was observed between the in vitro and in vivo periods, the in vitro period was correlated with chronotype, habitual sleep time, and preferred sleep time. Our data demonstrate that the in vitro period is significantly correlated with circadian/sleep preference. The findings suggest that fibroblasts from individual patients can be utilized for in vitro screening of therapeutic agents to provide personalized therapeutic regimens for CRSD patients.
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影响因子：
1.6
作者：
Kerkhof, GA
通讯作者：
Kerkhof, GA
影响因子：
3.7
作者：
Pagani L;Semenova EA;Moriggi E;Revell VL;Hack LM;Lockley SW;Arendt J;Skene DJ;Meier F;Izakovic J;Wirz-Justice A;Cajochen C;Sergeeva OJ;Cheresiz SV;Danilenko KV;Eckert A;Brown SA
通讯作者：
Brown SA
影响因子：
2.8
作者：
Noguchi T;Wang CW;Pan H;Welsh DK
通讯作者：
Welsh DK
影响因子：
5.5
作者：
MILLS, JN;MINORS, DS;WATERHOUSE, JM
通讯作者：
WATERHOUSE, JM
影响因子：
4.4
作者：
Koskenvuo, Markku;Hublin, Christer;Kaprio, Jaakko
通讯作者：
Kaprio, Jaakko