Variation among intact tissue samples reveals the core transcriptional features of human CNS cell classes.
Variation among intact tissue samples reveals the core transcriptional features of human CNS cell classes.
复制标题
完整的组织样品之间的变化揭示了人CNS细胞类别的核心转录特征。
DOI：
10.1038/s41593-018-0216-z
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发表时间：
2018-09
影响因子：
25
通讯作者：
Oldham MC
中科院分区：
文献类型：
--
作者：
Kelley KW;Nakao-Inoue H;Molofsky AV;Oldham MC
It is widely assumed that cells must be physically isolated to study their molecular profiles. However, intact tissue samples naturally exhibit variation in cellular composition, which drives covariation of cell-class-specific molecular features. By analyzing transcriptional covariation in 7221 intact CNS samples from 840 neurotypical individuals representing billions of cells, we reveal the core transcriptional identities of major CNS cell classes in humans. By modeling intact CNS transcriptomes as a function of variation in cellular composition, we identify cell-class-specific transcriptional differences in Alzheimer’s disease, among brain regions, and between species. Among these, we show that PMP2 is expressed by human but not mouse astrocytes and significantly increases mouse astrocyte size upon ectopic expression in vivo, causing them to more closely resemble their human counterparts. Our work is available as an online resource (http://oldhamlab.ctec.ucsf.edu/) and provides a generalizable strategy for determining the core molecular features of cellular identity in intact biological systems.
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影响因子：
9.8
作者：
Fraser HB;Khaitovich P;Plotkin JB;Pääbo S;Eisen MB
通讯作者：
Eisen MB
影响因子：
3.1
作者：
Benoit J;Ayoub A;Rakic P
通讯作者：
Rakic P
影响因子：
8.8
作者：
Fertuzinhos S;Li M;Kawasawa YI;Ivic V;Franjic D;Singh D;Crair M;Sestan N
通讯作者：
Sestan N
DOI：
10.1073/pnas.1507125112
发表时间：
2015-06-09
影响因子：
11.1
作者：
Darmanis S;Sloan SA;Zhang Y;Enge M;Caneda C;Shuer LM;Hayden Gephart MG;Barres BA;Quake SR
通讯作者：
Quake SR
人类肌肉和大脑代谢组的异常进化差异与人类认知和身体独特性相似
影响因子：
9.8
作者：
Bozek K;Wei Y;Yan Z;Liu X;Xiong J;Sugimoto M;Tomita M;Pääbo S;Pieszek R;Sherwood CC;Hof PR;Ely JJ;Steinhauser D;Willmitzer L;Bangsbo J;Hansson O;Call J;Giavalisco P;Khaitovich P
通讯作者：
Khaitovich P