Laminar and temporal expression dynamics of coding and noncoding RNAs in the mouse neocortex.
Laminar and temporal expression dynamics of coding and noncoding RNAs in the mouse neocortex.
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DOI：
10.1016/j.celrep.2014.01.036
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发表时间：
2014-03-13
期刊：
影响因子：
8.8
通讯作者：
Sestan N
中科院分区：
文献类型：
--
作者：
Fertuzinhos S;Li M;Kawasawa YI;Ivic V;Franjic D;Singh D;Crair M;Sestan N
大脑新皮质的标志是它分为六层，每一层都包含一组具有特征的细胞类型和突触连接。涉及板层发育和功能的转录事件仍然难以捉摸。在这里，我们使用了mRNA和小RNA物种的深度测序来深入了解小鼠初级体感新皮质出生后发育过程中各层之间的转录差异及其时间动态。一些新的编码和非编码转录本被鉴定出具有特定的时空表达和剪接模式。我们还确定了与不同生物过程相关的签名轨迹和基因共表达网络，以及这些过程之间的转录重叠。最后，我们提供了数据，使我们能够研究潜在的miRNA和mRNA的相互作用。总体而言，这项研究提供了小鼠新皮质中编码和非编码转录物的层流和时间表达动力学的综合视图，并为神经发育和转录组的研究提供了资源。
The hallmark of the cerebral neocortex is its organization into six layers, each containing a characteristic set of cell types and synaptic connections. The transcriptional events involved in laminar development and function still remain elusive. Here we employed deep sequencing of mRNA and small RNA species to gain insights into transcriptional differences among layers and their temporal dynamics during postnatal development of the mouse primary somatosensory neocortex. A number of novel coding and noncoding transcripts were identified with specific spatiotemporal expression and splicing patterns. We also identified signature trajectories and gene co-expression networks associated with distinct biological processes and transcriptional overlap between these processes. Finally, we provide data that allows the study of potential miRNA and mRNA interactions. Overall, this study provides an integrated view of the laminar and temporal expression dynamics of coding and noncoding transcripts in the mouse neocortex and a resource for studies of neurodevelopment and transcriptome.
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影响因子：
16.2
作者：
Espinosa JS;Stryker MP
通讯作者：
Stryker MP
影响因子：
7
作者：
Brooks, Angela N.;Yang, Li;Graveley, Brenton R.
通讯作者：
Graveley, Brenton R.
DOI：
10.1073/pnas.0509032102
发表时间：
2005-12-06
影响因子：
11.1
作者：
Chen, JG;Rasin, MR;Sestan, N
通讯作者：
Sestan, N
影响因子：
3.7
作者：
Fonta, C;Imbert, M
通讯作者：
Imbert, M
DOI：
10.1523/jneurosci.5222-10.2011
发表时间：
2011-05-18
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Callaway EM;Borrell V
通讯作者：
Borrell V