Overexpression of GPR41 attenuated glucose production in propionate-induced bovine hepatocytes.
Overexpression of GPR41 attenuated glucose production in propionate-induced bovine hepatocytes.
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DOI：
10.3389/fvets.2022.981640
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发表时间：
2022
影响因子：
3.2
通讯作者：
中科院分区：
文献类型：
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牛肝脏主要利用丙酸作为糖异生底物来合成葡萄糖。然而，丙酸盐对牛肝细胞葡萄糖产生的调节作用的机制仍鲜为人知。先前的研究已证明 G 蛋白偶联受体 41 (GPR41) 是丙酸受体。我们假设丙酸可能调节牛肝细胞中 GPR41 的葡萄糖产生。因此，本研究的目的是探讨丙酸和GPR41对牛肝细胞葡萄糖产生的调节作用。 GPR41 过度表达的肝细胞在 0 或 3 mM 丙酸盐存在下孵育 24 小时。这些结果表明，丙酸盐处理增强了参与糖异生的磷酸烯醇丙酮酸羧激酶2（PCK2）和丙酮酸羧化酶（PC）基因的表达（P < 0.01）。值得注意的是，丙酸盐的添加促进了牛肝细胞中葡萄糖的产生。与不存在丙酸相比，通过过表达质粒AAV1过表达GPR41的牛肝细胞中添加丙酸可增加GPR41的表达。有趣的是，丙酸盐在 GPR41 过表达的肝细胞中 PCK2 的表达显着减弱。重要的是，GPR41 的过度表达减弱了丙酸诱导的牛肝细胞中的葡萄糖输出。这些发现表明，GPR41 通过下调丙酸诱导的牛肝细胞中 PCK2 的表达来负向调节葡萄糖的产生。
Bovine liver mainly utilizes the propionate as a gluconeogenic substrate to synthesize the glucose. However, the mechanism underlying the regulatory effects of propionate on the glucose production in bovine hepatocytes remains less known. Previous studies have demonstrated G protein-coupled receptor 41 (GPR41) as receptors for propionate. We hypothesized that propionate may regulate the glucose production by GPR41 in bovine hepatocytes. Therefore, the aim of the study was to investigate the regulatory effects of propionate and GPR41 on glucose production in bovine hepatocytes. Hepatocytes with GPR41 overexpression were incubated in the presence of either 0 or 3 mM propionate for 24 h. These results showed that the expression of phosphoenolpyruvate carboxykinase 2 (PCK2) and pyruvate carboxylase (PC) genes involved in gluconeogenesis was enhanced (P < 0.01) with propionate treatment. Remarkably, the addition of propionate promotes the glucose production in bovine hepatocytes. Expression of GPR41 was increased by the addition of propionate in bovine hepatocytes overexpressed GPR41 by overexpression plasmid AAV1 compared with the absence of propionate. Interestingly, expression of PCK2 was markedly attenuated in GPR41 overexpressed-hepatocytes with propionate. Importantly, overexpression of GPR41 attenuated glucose output in propionate-induced bovine hepatocytes. These findings revealed that GPR41 negatively regulates glucose production by downregulating the expression of PCK2 in propionate-induced bovine hepatocytes.
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影响因子：
3.2
作者：
Yang T;Ma X;Jiang M;Cheng Z;Datsomor O;Zhao G;Zhan K
通讯作者：
Zhan K
DOI：
10.1073/pnas.2637002100
发表时间：
2004-01-27
影响因子：
11.1
作者：
Xiong, YM;Miyamoto, N;Yanagisawa, M
通讯作者：
Yanagisawa, M
影响因子：
3.5
作者：
Zhan, Kang;Yang, Tian Yu;Zhao, Guo Qi
通讯作者：
Zhao, Guo Qi
影响因子：
82.9
作者：
Miller RA;Shi Y;Lu W;Pirman DA;Jatkar A;Blatnik M;Wu H;Cárdenas C;Wan M;Foskett JK;Park JO;Zhang Y;Holland WL;Rabinowitz JD;Birnbaum MJ
通讯作者：
Birnbaum MJ
影响因子：
29
作者：
Li, Erwei;Shan, Haili;Wang, Yiguo
通讯作者：
Wang, Yiguo