The Role of Tea Tree Oil in Alleviating Palmitic Acid-Induced Lipid Accumulation in Bovine Hepatocytes.
The Role of Tea Tree Oil in Alleviating Palmitic Acid-Induced Lipid Accumulation in Bovine Hepatocytes.
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DOI：
10.3389/fvets.2021.814840
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发表时间：
2021
影响因子：
3.2
通讯作者：
Zhan K
中科院分区：
文献类型：
--
作者：
Yang T;Ma X;Jiang M;Cheng Z;Datsomor O;Zhao G;Zhan K
茶树油 (TTO) 在脂质代谢中发挥着重要作用，可减轻炎症反应。脂肪肝与肝细胞中的脂质积累有关，导致炎症。然而，关于 TTO 对牛肝细胞脂质积累和炎症影响的信息非常有限。本研究旨在评估 TTO 是否可以减轻棕榈酸 (PA) 诱导的牛肝细胞脂质积累。从泌乳中期荷斯坦奶牛中分离出的肝细胞用 100 μM PA 预处理 72 小时。细胞单独用 PA 预处理（PA 组）或用 PA 后用 0.00625% TTO 处理 12 小时（PT 组）。与PA组相比，PT组脂肪酸氧化基因表达增加（P < 0.05），脂肪酸合成基因表达减少（P < 0.05）。 PA 治疗导致肿瘤坏死因子-α (TNF-α) 和白细胞介素 6 (IL-6) 表达增加 (P < 0.05)，但 PT 组的增加幅度较小 (P < 0.05)。与PA组相比，TTO处理抑制了磷酸化(p)-p65和p-抑制剂κBα(p-IκBα)的表达(P < 0.05)。 TTO 处理限制 (P < 0.05) 细胞内活性氧 (ROS) 的增加，并防止 (P < 0.05) PA 处理引起的线粒体膜电位降低。与PA组相比，PT组内质网（ER）应激基因表达降低（P < 0.05）。我们的结果表明，TTO 治疗减弱了 PA 在肝细胞中的作用，导致脂肪酸氧化、减少脂肪酸合成、抑制炎症反应并减少 ER 应激。总而言之，这项研究的结果表明，TTO 治疗可能是一种有前途的治疗方法，可治疗奶牛产犊前后不久的脂质稳态失衡、炎症和内质网应激。
Tea tree oil (TTO) plays an important role in lipid metabolism, alleviating the inflammatory responses. Fatty liver is associated with lipid accumulation in hepatocytes, leading to inflammation. However, there is very limited information on the effects of TTO on lipid accumulation, and inflammation in bovine hepatocytes. This study aimed to evaluate whether TTO alleviates palmitic acid (PA)-induced lipid accumulation in bovine hepatocytes. Hepatocytes isolated from mid-lactating Holstein cows were pretreated with 100 μM PA for 72 h. Cells were either pretreated with PA alone (PA group) or with PA followed by 0.00625% TTO treatment for 12 h (PT group). Expression of fatty acid oxidant genes increased (P < 0.05) while fatty acid synthesis genes decreased (P < 0.05) in the PT group compared with the PA group. PA treatment resulted in increased (P < 0.05) expression of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), but these increases were less in the PT group (P < 0.05). Compared to the PA group, expression of phosphorylated (p)-p65 and p-inhibitor κBα (p-IκBα) was suppressed (P < 0.05) by TTO treatment. TTO treatment limited (P < 0.05) the increase in intracellular reactive oxygen species (ROS) and prevented (P < 0.05) a reduction in mitochondrial membrane potential observed in response to PA treatment. Expression of endoplasmic reticulum (ER) stress genes was reduced (P < 0.05) in the PT group compared with the PA group. Our results suggest that TTO treatment attenuates the effects of PA in hepatocytes, leading to fatty acid oxidation, decreased fatty acid synthesis, suppressed inflammatory response, and reduced ER stress. Taken together, the results of this study suggest that TTO treatment may be a promising therapeutic approach to imbalanced lipid homeostasis, inflammation and ER stress in dairy cows shortly before and after calving.
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影响因子：
4.2
作者：
Fu Y;Jin Y;Shan A;Zhang J;Tang H;Shen J;Zhou C;Yu H;Fang H;Zhao Y;Wang J;Tian Y
通讯作者：
Tian Y
DOI：
10.3390/ani11082460
发表时间：
2021-08-21
期刊：
Animals : an open access journal from MDPI
影响因子：
--
作者：
Hu Z;Lin M;Ma X;Zhao G;Zhan K
通讯作者：
Zhan K
影响因子：
3.5
作者：
Gross, J. J.;Schwarz, F. J.;Bruckmaier, R. M.
通讯作者：
Bruckmaier, R. M.
影响因子：
5.3
作者：
Lecomte, Virginie;Meugnier, Emmanuelle;Lefai, Etienne
通讯作者：
Lefai, Etienne
影响因子：
6.7
作者：
Brand, C;Townley, SL;Hart, PH
通讯作者：
Hart, PH