Carotenoid Nostoxanthin Production by Sphingomonas sp. SG73 Isolated from Deep Sea Sediment.
Carotenoid Nostoxanthin Production by Sphingomonas sp. SG73 Isolated from Deep Sea Sediment.
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DOI：
10.3390/md19050274
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发表时间：
2021-05-14
期刊：
影响因子：
5.4
通讯作者：
Hara KY
中科院分区：
文献类型：
--
作者：
Kikukawa H;Okaya T;Maoka T;Miyazaki M;Murofushi K;Kato T;Hirono-Hara Y;Katsumata M;Miyahara S;Hara KY
类胡萝卜素由于其抗氧化活性而在商业上用于膳食补充剂、化妆品和药物。本研究从日本静冈骏河湾采集的深海沉积物中分离出有色微生物。其中一个菌株被发现是一个纯的黄色类胡萝卜素生产商，该菌株被确定为鞘氨醇单胞菌属（变形菌门）的16 S rRNA基因序列分析;该属的成员通常是从空气中分离，人体，和海洋环境。经质谱（MS）、质谱/质谱（MS/MS）和紫外-可见吸收光谱（UV-Vis）分析，确定其为（2，3，2 ′，3 ′）-β，β-胡萝卜素-2，3，2 ′，3 ′-四醇（nostoxanthin，（2，3，2 ′，3 ′）-β-carotene-2，3，2 ′，3 ′-tetrol）。Nostoxanthin是一种多羟基黄色类胡萝卜素，分离自一些光合细菌，包括一些种类的蓝细菌。菌株Sphingomonassp.SG73产生高纯度的nostoxanthin的总类胡萝卜素产量的约97%（面积%），该菌株是嗜盐和耐受1.5倍高的盐浓度相比，海水。当在1.8%的人工海盐中生长时，与没有人工海盐的生产相比，nostoxanthin的产量增加了2.5倍。这些结果表明，鞘氨醇单胞菌SG 73是一个有效的生产念珠藻黄素，该菌株是理想的，因为它与海盐的兼容性，利用海水生产类胡萝卜素。
Carotenoids are used commercially for dietary supplements, cosmetics, and pharmaceuticals because of their antioxidant activity. In this study, colored microorganisms were isolated from deep sea sediment that had been collected from Suruga Bay, Shizuoka, Japan. One strain was found to be a pure yellow carotenoid producer, and the strain was identified as Sphingomonas sp. (Proteobacteria) by 16S rRNA gene sequence analysis; members of this genus are commonly isolated from air, the human body, and marine environments. The carotenoid was identified as nostoxanthin ((2,3,2′,3′)-β,β-carotene-2,3,2′,3′-tetrol) by mass spectrometry (MS), MS/MS, and ultraviolet-visible absorption spectroscopy (UV-Vis). Nostoxanthin is a poly-hydroxy yellow carotenoid isolated from some photosynthetic bacteria, including some species of Cyanobacteria. The strain Sphingomonas sp. SG73 produced highly pure nostoxanthin of approximately 97% (area%) of the total carotenoid production, and the strain was halophilic and tolerant to 1.5-fold higher salt concentration as compared with seawater. When grown in 1.8% artificial sea salt, nostoxanthin production increased by 2.5-fold as compared with production without artificial sea salt. These results indicate that Sphingomonas sp. SG73 is an efficient producer of nostoxanthin, and the strain is ideal for carotenoid production using marine water because of its compatibility with sea salt.
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影响因子：
5.4
作者：
Kumar SR;Hosokawa M;Miyashita K
通讯作者：
Miyashita K
影响因子：
5.4
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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