Immunochemical characterization of adenocarcinoma‐associated antigen yh206
Immunochemical characterization of adenocarcinoma‐associated antigen yh206
复制标题
腺癌相关抗原 yh206 的免疫化学表征
DOI：
--
复制
发表时间：
1988
影响因子：
6.4
通讯作者：
A. Yachi
中科院分区：
文献类型：
--
作者：
Y. Hinoda;K. Imai;T. Ban;T. Endo;A. Yachi
由MAb YH 206识别的抗原主要在腺癌中表达，并且也在癌症患者的血清中检测到（Hinoda等人，1987年）。该抗原（抗原YH 206）进行了化学表征，并通过柱层析纯化。SDS-PAGE分析显示了高分子量范围内的广泛组分，其可通过碳水化合物（PAS）而非蛋白质（银）染色明确检出。抗原YH 206的琼脂糖凝胶电泳和免疫印迹表明，它由高分子量组分（超过2，000 kDa）组成。用碱处理抗原YH 206，提示抗原决定簇由粘蛋白型糖链组成。密度梯度电泳显示抗原YH 206的活性定位于1.45 μ g/ml的密度，表明抗原YH 206是粘蛋白。神经氨酸酶处理抗原YH 206表明，该表位是隐蔽的，含有一个无唾液酸碳水化合物链。一旦抗原YH 206通过亲和色谱法纯化，则CA 19 - 9抗原和DU-PAN-II抗原均不能检测到，尽管它们在粗腹水起始材料中以非常高的水平存在;后两种是广泛用于血清诊断以检测腺癌的代表性碳水化合物抗原。
The antigen recognized by MAb YH206 is mainly expressed in adenocarcinomas and is also detected in the sera of cancer patients (Hinoda et al., 1987). This antigen (antigen YH206) was chemically characterized and purified by column chromatography. SDS‐PAGE analysis revealed a broad component in the high‐molecular‐weight range which was clearly detectable by carbohydrate (PAS) but not by protein (silver) stain. Agarose gel electrophoresis and immunoblotting of antigen YH206 indicated that it consists of a high‐molecular‐weight component (more than 2,000 kDa). Treatment of antigen YH206 with alkali suggested that the antigenic determinant consists of carbohydrate chains of mucin type. Density gradient ultracentrifugation revealed that the activity of antigen YH206 is localized at a density of 1.45 g/ml, suggesting that antigen YH206 is a mucin. Neuraminidase treatment of antigen YH206 indicated that the epitope is cryptic and contains an asialocarbohydrate chain. Once antigen YH206 has been purified by affinity chromatography, neither CA 19‐9 antigen nor DU‐PAN‐II antigen can be detected, although they were present at very high levels in the crude ascitic starting material; these last two are representative carbohydrate antigens which are widely used for serodiagnosis to detect adenocarcinomas.
影响因子：
56.9
作者：
KOPROWSKI, H;HERLYN, M;SEARS, HF
通讯作者：
SEARS, HF
影响因子：
11.2
作者：
K. Fukushima;M. Hirota;P. Terasaki;A. Wakisaka;H. Togashi;D. Chia;N. Suyama;Y. Fukushi;E. Nudelman;S. Hakomori
通讯作者：
K. Fukushima;M. Hirota;P. Terasaki;A. Wakisaka;H. Togashi;D. Chia;N. Suyama;Y. Fukushi;E. Nudelman;S. Hakomori
双岩藻神经节苷脂 (VI3NeuAcV3III3Fuc2nLc6) 在正常和肿瘤组织中的位置和分布由其单克隆抗体 FH6 确定。
影响因子：
11.2
作者：
Fukushi,Y;Kannagi,R;Hakomori,S;Shepard,T;Kulander,BG;Singer,JW
通讯作者：
Singer,JW