Self-Assembly of Recombinant Silk as a Strategy for Chemical-Free Formation of Bioactive Coatings: A Real-Time Study.
Self-Assembly of Recombinant Silk as a Strategy for Chemical-Free Formation of Bioactive Coatings: A Real-Time Study.
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重组丝的自组装作为无化学形成生物活性涂层的策略：实时研究。
DOI：
10.1021/acs.biomac.6b01721
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发表时间：
2017
影响因子：
6.2
通讯作者：
M. Hedhammar
中科院分区：
文献类型：
--
作者：
Linnea Nilebäck;J. Hedin;M. Widhe;L. Floderus;Annika Krona;H. Bysell;M. Hedhammar
利用生物活性肽对生物材料进行功能化，可以提高其植入后的性能。通过与自组装蛋白的基因融合，功能肽可以很容易地以不同的物理形式呈现。本文描述了一种基于重组蜘蛛丝蛋白4RepCT自组装的无化学涂层方法，并用于在各种生物材料表面制备功能涂层。实时研究了丝蛋白的组装过程，揭示了丝蛋白在表面连续组装的发生和纳米纤维结构的形成。对吸附量和粘弹性进行了评价，结果表明，该涂层在氯化氢、氢氧化钠和乙醇洗涤下是稳定的。钛、不锈钢和羟基磷灰石被丝质包裹，丝质与抗菌肽或纤连蛋白基序融合。在功能性蚕丝涂层上培养的人原代细胞表现出良好的细胞活力和增殖能力，这意味着有可能提高植入物的性能和人体的接受度。
Functionalization of biomaterials with biologically active peptides can improve their performance after implantation. By genetic fusion to self-assembling proteins, the functional peptides can easily be presented on different physical formats. Herein, a chemical-free coating method based on self-assembly of the recombinant spider silk protein 4RepCT is described and used to prepare functional coatings on various biomaterial surfaces. The silk assembly was studied in real-time, revealing the occurrence of continuous assembly of silk proteins onto surfaces and the formation of nanofibrillar structures. The adsorbed amounts and viscoelastic properties were evaluated, and the coatings were shown to be stable against wash with hydrogen chloride, sodium hydroxide, and ethanol. Titanium, stainless steel, and hydroxyapatite were coated with silk fused to an antimicrobial peptide or a motif from fibronectin. Human primary cells cultured on the functional silk coatings show good cell viability and proliferation, implying the potential to improve implant performance and acceptance by the body.
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影响因子：
14
作者：
Raphel J;Holodniy M;Goodman SB;Heilshorn SC
通讯作者：
Heilshorn SC
影响因子：
9.7
作者：
Vidal, Guillaume;Blanchi, Thomas;Mieszawska, Aneta J.;Calabrese, Rossella;Rossi, Claire;Vigneron, Pascale;Duval, Jean-Luc;Kaplan, David L.;Egles, Christophe
通讯作者：
Egles, Christophe
影响因子：
14
作者：
Raphel J;Karlsson J;Galli S;Wennerberg A;Lindsay C;Haugh MG;Pajarinen J;Goodman SB;Jimbo R;Andersson M;Heilshorn SC
通讯作者：
Heilshorn SC
影响因子：
64.8
作者：
Hagn, Franz;Eisoldt, Lukas;Kessler, Horst
通讯作者：
Kessler, Horst