Enhanced cellular adhesion on titanium by silk functionalized with titanium binding and RGD peptides.
Enhanced cellular adhesion on titanium by silk functionalized with titanium binding and RGD peptides.
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DOI：
10.1016/j.actbio.2012.09.003
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发表时间：
2013-01
影响因子：
9.7
通讯作者：
Egles, Christophe
中科院分区：
文献类型：
--
作者：
Vidal, Guillaume;Blanchi, Thomas;Mieszawska, Aneta J.;Calabrese, Rossella;Rossi, Claire;Vigneron, Pascale;Duval, Jean-Luc;Kaplan, David L.;Egles, Christophe
钛上的软组织粘连对可植入材料来说是一个挑战。为了改善细胞/材料界面的粘附性，我们采用了一种基于特定多肽对钛分子识别的新方法。将丝素蛋白化学接枝到钛结合多肽(TiBP)上，以增加这些嵌合蛋白在金属表面的吸附。用石英晶体微天平定量了TiBP功能化丝绸的特定吸附，结果表明，由于结合肽的存在，蛋白质沉积增加了35%以上。然后将TiBP和纤维连接蛋白衍生的RGD多肽接枝到丝蛋白上，制备了丝蛋白。成纤维细胞在多功能丝绸涂层修饰的钛表面的黏附实验表明，黏附细胞的数量增加了60%。扫描电子显微镜和焦点接触点免疫细胞化学染色显示粘附性增强。鸡胚器官培养也显示在多功能丝肽涂层上扩张的内皮细胞具有很强的粘附性。这些结果表明，TiBP和RGD功能化的Silk是一种很有前途的细胞-生物材料界面修饰方法，为植入式医疗设备开辟了新的前景，特别是在需要再内皮化的情况下。
Soft tissue adhesion on titanium represents a challenge for implantable materials. In order to improve adhesion at the cell/material interface we used a new approach based on the molecular recognition of titanium by specific peptides. Silk fibroin protein was chemically grafted with titanium binding peptide (TiBP) to increase adsorption of these chimeric proteins to the metal surface. Quartz Crystal Microbalance was used to quantify the specific adsorption of TiBP-functionalized silk and an increase in protein deposition by more than 35% was demonstrated due to the presence of the binding peptide. A silk protein grafted with TiBP and fibronectin-derived RGD peptide was then prepared. The adherence of fibroblasts on the titanium surface modified with the multifunctional silk coating demonstrated an increase in the number of adhering cells by 60%. The improved adhesion was demonstrated by Scanning Electron Microscopy and immunocytochemical staining of focal contact points. Chick embryo organotypic culture also revealed strong adhesion of endothelial cells expanding on the multifunctional silk-peptide coating. These results demonstrated that silk functionalized with TiBP and RGD represents a promising approach to modify cell-biomaterial interfaces, opening new perspectives for implantable medical devices, especially when reendothelialization is required.
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影响因子：
3.9
作者：
Chen, Haibin;Su, Xiaodi;Choe, Woo-Seok
通讯作者：
Choe, Woo-Seok
影响因子：
14
作者：
IKADA, Y
通讯作者：
IKADA, Y
影响因子：
14
作者：
Murphy, Amanda R.;John, Peter St.;Kaplan, David L.
通讯作者：
Kaplan, David L.
影响因子：
14
作者：
Panilaitis, B;Altman, GH;Kaplan, DL
通讯作者：
Kaplan, DL
影响因子：
14
作者：
Altman, GH;Diaz, F;Kaplan, DL
通讯作者：
Kaplan, DL