Ionic basis of a mechanotransduction current in adult rat dorsal root ganglion neurons.
Ionic basis of a mechanotransduction current in adult rat dorsal root ganglion neurons.
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DOI：
10.1186/1744-8069-2-28
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发表时间：
2006-08-21
期刊：
影响因子：
3.3
通讯作者：
Levine JD
中科院分区：
文献类型：
--
作者：
McCarter GC;Levine JD
听觉、本体感觉、触觉和痛觉所必需的感觉机械传导仍然知之甚少。在躯体感觉中，即使是被认为介导机械转导的机械敏感的兴奋性离子电流的基本性质也在很大程度上未被描述。我们记录了由压电驱动的玻璃探针撞击离体大鼠背根神经节（DRG）神经元的索马体所引起的全细胞离子电流。几乎在所有测试的DRG神经元中观察到这种瞬时机械激活电流。在离子取代实验中，电流可以被大多数阳离子非选择性地携带，包括二价阳离子和有机阳离子，但不能被氯离子或硫酸根离子携带。此外，机械活化的电流进行的一价阳离子始终阻止由外部钙或镁的毫摩尔浓度。基于这些结果，在体外躯体感觉神经元中观察到的瞬时机械转导电流是由对阳离子无选择性但对阴离子不可渗透的大孔机械门控通道介导。
Sensory mechanical transduction – necessary for hearing, proprioception, and the senses of touch and pain – remains poorly understood. In somatosensation, even the basic properties of the mechanically sensitive excitatory ionic currents that are assumed to mediate mechanical transduction are largely undescribed. We have recorded, from the soma of rat dorsal root ganglion (DRG) neurons in vitro, whole-cell ionic currents induced by the impact of a piezo-electrically driven glass probe. This transient mechanically activated current was observed in virtually all DRG neurons tested. In ion substitution experiments the current could be carried nonselectively by most cations, including divalent and organic cations, but not by chloride or sulfate ions. In addition, the mechanically activated current carried by monovalent cations was consistently blocked by millimolar concentrations of external calcium or magnesium. Based on these results, the transient mechanical transduction current observed in somatosensory neurons in vitro is mediated by large-pore mechanically gated channels nonselective for cations but impermeable to anions.
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影响因子：
5.3
作者：
Cho, HW;Shin, J;Oh, U
通讯作者：
Oh, U
DOI：
10.1073/pnas.0508005103
发表时间：
2006-03-21
影响因子：
11.1
作者：
Di Castro, A;Drew, LJ;Cesare, P
通讯作者：
Cesare, P
影响因子：
5.3
作者：
Drew, LJ;Wood, JN;Cesare, P
通讯作者：
Cesare, P
影响因子：
2.5
作者：
KRESS, M;KOLTZENBURG, M;HANDWERKER, HO
通讯作者：
HANDWERKER, HO
影响因子：
2.5
作者：
McCarter, GC;Reichling, DB;Levine, JD
通讯作者：
Levine, JD