Development of a recombinant truncated nucleocapsid protein based immunoassay for detection of antibodies against human coronavirus OC43.
Development of a recombinant truncated nucleocapsid protein based immunoassay for detection of antibodies against human coronavirus OC43.
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DOI：
10.1016/j.jviromet.2011.07.005
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发表时间：
2011-10
影响因子：
3.1
通讯作者：
Haynes LM
中科院分区：
文献类型：
--
作者：
Blanchard EG;Miao C;Haupt TE;Anderson LJ;Haynes LM
HCoV-OC 43截短型N免疫检测的敏感性和特异性分别为90.9%（10/11）和82.9%（39/47）。该检测方法与以前的检测方法的诊断符合率为100%。该方法可作为冠状病毒血清流行病学研究的一个有用工具。人类冠状病毒是人类上呼吸道感染的主要原因之一。虽然它们更经常导致轻微疾病，但它们与需要住院治疗的疾病有关。在这项研究中，使用截短的重组核衣壳（N）蛋白抗原在酶免疫吸附试验（ELISA）中开发了一种用于人类冠状病毒之一OC 43的检测方法，并使用从HCoV-OC 43感染患者、健康成人和其他呼吸道病毒感染患者中采集的血清进行了评价。结果表明，该方法诊断乳腺癌的敏感性为90.9%（10/11），特异性为82.9%（39/47）。为了评估ELISA的临床效用，筛选在HCoV-OC 43感染爆发期间从患者收集的并且先前通过HCoV-OC 43全N ELISA鉴定为阳性的血清样品，导致测试方法之间的100%诊断一致性。这些结果表明，该检测方法提供了一个可靠的方法来检测HCoV-OC 43感染，并可能是一个有用的工具，在冠状病毒血清流行病学研究。
► The diagnostic sensitivity and specificity of the HCoV-OC43 truncated N immunoassay were 90.9% (10/11) and 82.9% (39/47), respectively. ► The assay demonstrated 100% diagnosis agreement with the previous testing methods. ► This assay offers may be a useful tool in coronavirus seroepidemiological studies. Human coronaviruses are one of the main causes of upper respiratory tract infections in humans. While more often responsible for mild illness, they have been associated with illnesses that require hospitalization. In this study, an assay for one of the human coronaviruses, OC43, was developed using a truncated recombinant nucleocapsid (N) protein antigen in an enzyme immunosorbent assay (ELISA) and evaluated using serum collected from HCoV-OC43-infected patients, healthy adults, and patients with other respiratory virus infections. Results showed that the diagnostic sensitivity and specificity of the assay were 90.9% (10/11) and 82.9% (39/47), respectively. To evaluate the clinical utility of the ELISA, serum samples collected from patients during an outbreak of HCoV-OC43 infection and previously identified as positive by HCoV-OC43 whole N ELISA were screened resulting in 100% diagnosis agreement between the testing methods. These results suggest that this assay offers a reliable method to detect HCoV-OC43 infection and may be a useful tool in coronavirus seroepidemiological studies.
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