ATP-competitive mTOR kinase inhibitors delay plant growth by triggering early differentiation of meristematic cells but no developmental patterning change.
ATP-competitive mTOR kinase inhibitors delay plant growth by triggering early differentiation of meristematic cells but no developmental patterning change.
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ATP竞争力MTOR激酶抑制剂通过触发分生细胞的早期分化而延迟植物的生长，但没有发育模式变化。
DOI：
10.1093/jxb/ert242
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发表时间：
2013-11
影响因子：
6.9
通讯作者：
Menand B
中科院分区：
文献类型：
--
作者：
Montané MH;Menand B
TOR（雷帕霉素靶蛋白）蛋白，一种在真核生物中保守的大型磷脂酰肌醇3-激酶样蛋白激酶（PIKK），是生长和代谢的中心调节因子。由于植物对雷帕霉素的敏感性很低，因此对TOR在植物生长发育中的作用的研究受到限制。由于TOR的激酶结构域是高度保守的，本研究分析了三组第一代和第二代ATP竞争性抑制剂（称为活性位点TOR抑制剂的asTORIS）最近开发的人TOR激酶对拟南芥生长的剂量依赖性影响。所有六种asTORis都以剂量依赖性方式抑制植物根的生长，第一代和第二代抑制剂的50%生长抑制剂量（GI 50）分别为<10 μM和<1 μM，与哺乳动物细胞中的值相似。遗传学方法进一步证明，只有asTOR抑制根生长的AtTOR基因剂量依赖性的方式。AsTORis减少了以下的长度：（i）分生区（MZ）;（ii）MZ中的分裂区;（iii）伸长区中的表皮细胞;和（iv）根毛细胞。而分生组织细胞致力于早期分化，细胞分化的模式本身不受影响。通过使用其他生长抑制剂阻断细胞周期或翻译，显示AsTORis诱导的根毛生长表型是特异性的。在不同的开花植物组中也观察到AsTORis对生长和根毛的剂量依赖性抑制，表明asTORis可用于研究其他被子植物（包括作物）中的TOR途径。
The TOR (target of rapamycin) protein, a large phosphatidylinositol 3-kinase-like protein kinase (PIKK) that is conserved in eukaryotes and is a central regulator of growth and metabolism. The analysis of function of TOR in plant growth and development has been limited by the fact that plants are very poorly sensitive to rapamycin. As the kinase domain of TOR is highly conserved, this study analysed the dose-dependent effect of three sets of first- and second-generation ATP-competitive inhibitors (called asTORis for active-site TOR inhibitors) recently developed for the human TOR kinase on Arabidopsis thaliana growth. All six asTORis inhibited plant root growth in a dose-dependent manner, with 50% growth inhibitory doses (GI50) of <10 μM and <1 μM for the first- and second-generation inhibitors, respectively, similarly to the values in mammalian cells. A genetic approach further demonstrated that only asTORis inhibited root growth in an AtTOR gene-dosage-dependent manner. AsTORis decreased the length of: (i) the meristematic zone (MZ); (ii) the division zone in the MZ; (iii) epidermal cells in the elongation zone; and (iv) root hair cells. Whereas meristematic cells committed to early differentiation, the pattern of cell differentiation was not affected per se. AsTORis-induced root hair growth phenotype was shown to be specific by using other growth inhibitors blocking the cell cycle or translation. AsTORis dose-dependent inhibition of growth and root hairs was also observed in diverse groups of flowering plants, indicating that asTORis can be used to study the TOR pathway in other angiosperms, including crop plants.
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影响因子：
7.4
作者：
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