Hyperthermia-enhanced indocyanine green delivery for laser-induced thermal ablation of carcinomas.
Hyperthermia-enhanced indocyanine green delivery for laser-induced thermal ablation of carcinomas.
复制标题
DOI：
10.3109/02656736.2013.817615
复制
发表时间：
2013-08
期刊：
影响因子：
--
通讯作者：
Griffin RJ
中科院分区：
文献类型：
--
作者：
Barnes KD;Shafirstein G;Webber JS;Koonce NA;Harris Z;Griffin RJ
静脉注射吲哚菁绿色（ICG）染料可以有效地将近红外激光转化为热量并增强血管的热损伤;然而，与其他组织相比，肿瘤对ICG没有选择性摄取，这影响了该策略的治疗率，除非肿瘤组织中的肿瘤摄取以某种方式增加。在这里，我们研究了在静脉注射ICG之前使用局部热疗来增强肿瘤组织中的ICG摄取，从而对实体瘤进行激光热消融。用755 nm激光治疗SCK或SCCVII肿瘤的雌性A/J和C3 H小鼠，使用70 J/cm 2辐射暴露和3 ms脉冲。激光治疗单独进行或与先前静脉内施用4 mg/kg ICG一起进行，在42.5°C下进行或不进行局部肿瘤热疗60分钟。使用近红外动物成像系统定量ICG的保留。对组织进行组织学检查以确定损伤。在两种模型中，加热肿瘤中的ICG摄取和保留平均比非加热肿瘤高1.23倍。在SCK肿瘤中，单独接受激光照射或与ICG联合接受激光照射的动物肿瘤生长延迟分别增加了1.86和3.91倍。在ICG注射前增加局部热疗导致SCK肿瘤完全消退。尽管SCCVII肿瘤中ICG的摄取增加，但观察到肿瘤生长延迟的变化很小。使用局部热疗可以改善ICG向肿瘤的递送，从而增加可以有效暴露于激光治疗的较小浅表恶性肿瘤的激光热消融的程度。
Intravenous administration of indocyanine green (ICG) dye can effectively convert near infrared laser light into heat and enhance thermal injury of blood vessels; however, there is no selective uptake of ICG by the tumor compared to the other tissues which impacts the therapeutic ratio of this stratefy unless tumor uptake is somehow increases in tumor tissue. Here, we investigated the use of local hyperthermia prior to intravenous ICG administration to enhance ICG uptake in tumor tissue and thereby laser thermal ablation of solid tumors. Female A/J and C3H mice with SCK or SCCVII tumors were treated with 755-nm laser light using 70 J/cm2 radiant exposures and a 3 ms pulse. The laser therapy was performed either alone or with prior intravenous administration of 4 mg/kg ICG with and without local tumor hyperthermia at 42.5°C for 60 minutes. Retention of ICG was quantified using a near-infrared animal imaging system. Tissues were examined for damage histologically. The ICG uptake and retention in the heated tumors was 1.23-fold greater on average compared to non-heated tumors, in both models. In SCK tumors, animals receiving either laser irradiation alone or in conjunction with ICG had 1.86 and 3.91-fold increase in tumor growth delay, respectively. The addition of local hyperthermia before ICG injection resulted in complete regression of SCK tumors. Although uptake of ICG was increased in SCCVII tumors, little change in tumor growth delay was observed. Using local hyperthermia may improve the delivery of ICG to the tumor and thereby increase the extent of laser thermal ablation of smaller superficial malignancies that can be effectively exposed to laser therapy.
登录
查看更多内容
影响因子：
6.2
作者：
Zafereo, Mark E.;Hanasono, Matthew M.;Weber, Randal S.
通讯作者：
Weber, Randal S.
DOI：
10.1016/1010-6030(95)04292-x
发表时间：
1996-05-31
影响因子：
4.3
作者：
Philip, R;Penzkofer, A;Abels, C
通讯作者：
Abels, C
影响因子：
10.3
作者：
Dreher, MR;Liu, WG;Chilkoti, A
通讯作者：
Chilkoti, A
影响因子：
3.4
作者：
Chang, CC;Sun, W;Trosko, JE
通讯作者：
Trosko, JE
影响因子：
6.4
作者：
Shafirstein, Gal;Baeumler, Wolfgang;Hennings, Leah J.;Siegel, Eric R.;Friedman, Ran;Moreno, Mauricio A.;Webber, Jessica;Jackson, Cassie;Griffin, Robert J.
通讯作者：
Griffin, Robert J.