Indocyanine green enhanced near-infrared laser treatment of murine mammary carcinoma.
Indocyanine green enhanced near-infrared laser treatment of murine mammary carcinoma.
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DOI：
10.1002/ijc.26126
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发表时间：
2012-03-01
影响因子：
6.4
通讯作者：
Griffin, Robert J.
中科院分区：
文献类型：
--
作者：
Shafirstein, Gal;Baeumler, Wolfgang;Hennings, Leah J.;Siegel, Eric R.;Friedman, Ran;Moreno, Mauricio A.;Webber, Jessica;Jackson, Cassie;Griffin, Robert J.
近红外（NIR）激光适用于消融良性病变并在深层血管中引起不可逆的热损伤，这一点已被广泛接受。在此波长下，激光穿透皮肤深处（3-5 mm）。然而，许多研究人员报告说，在近红外激光治疗期间和治疗后立即出现明显的疼痛，从轻微到严重。静脉注射外源性发色团（吲哚菁绿色，ICG，染料）可有效地将NIR激光转化为热量。在这种方法中，ICG的存在已显示在健康组织的治疗中增强血管的热损伤。然而，热损伤对ICG/NIR激光治疗期间皮肤癌消退的有效性尚未评估。本研究的目的是评估使用ICG/NIR激光治疗热损伤浅表癌的潜在益处。用808-nm NIR激光照射两组具有皮下乳腺腺癌肿瘤（7- 9mm）的A/J小鼠，然后尾静脉注射ICG染料或无菌盐水。皮下组织的组织学评价显示，激光/生理盐水组有轻微的热损伤和坏死，激光/ICG组有实质性损伤（高达100%坏死）。与激光/生理盐水组相比，激光/ICG治疗组显示肿瘤体积稳定减小：第5天为48%（P=0.045），第8、9和10天为69%-70%（P值0.0005或更低）。血管靶向ICG-NIR激光治疗似乎具有治疗浅表肿瘤的潜力。
It is well accepted that near-infrared (NIR) lasers are appropriate to ablate benign lesions and induce irreversible thermal injury in deeply seated blood vessels. At this wavelength, the laser light penetrates deep (3–5 mm) into the skin. However, many researchers have reported noticeable pain, extending from mild to severe, during and immediately following NIR laser treatment. Intravenous administration of an exogenous chromophore (indocyanine green, ICG, dye) can effectively convert NIR laser light into heat. In this approach the presence of ICG has shown to enhance thermal injury of blood vessels in the treatment of healthy tissues. However, the effectiveness of thermal injury on the regression of cutaneous carcinomas during ICG/NIR laser therapy has not been assessed. The purpose of this study was to evaluate the potential benefit of using ICG/NIR laser therapy to regress superficial carcinoma with thermal injury. Two groups of A/J mice with subcutaneous mammary adenocarcinoma tumors (7–9 mm) were irradiated with a 808-nm NIR laser preceded by tail vein injection of ICG dye or sterile saline. Histological evaluation of the subcutaneous tissue revealed minor thermal damage and necrosis in the laser/saline group and substantial damage (up to 100% necrosis) in the laser/ICG group. The laser/ICG-treated group showed a steady reduction in tumor volume compared to the laser/saline group: 48% by Day 5 (P=0.045) and 69%–70% by Days 8, 9, and 10 (P values 0.0005 or less). The vascular targeted ICG–NIR laser therapy appears to have potential for treating superficial tumors.
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DOI：
10.1016/1010-6030(95)04292-x
发表时间：
1996-05-31
影响因子：
4.3
作者：
Philip, R;Penzkofer, A;Abels, C
通讯作者：
Abels, C
影响因子：
2.6
作者：
Griffin, RJ;Okajima, K;Song, CW
通讯作者：
Song, CW
影响因子：
3.4
作者：
Chang, CC;Sun, W;Trosko, JE
通讯作者：
Trosko, JE
影响因子：
6.1
作者：
HALLER, M;AKBULUT, C;PETER, K
通讯作者：
PETER, K
影响因子：
4.6
作者：
Hetz, Hubert;Faybik, Peter;Krenn, Claus Georg
通讯作者：
Krenn, Claus Georg