Neuropeptide Y receptor Y2 (npy2r) deficiency reduces anxiety and increases food intake in Japanese medaka (Oryzias latipes).
Neuropeptide Y receptor Y2 (npy2r) deficiency reduces anxiety and increases food intake in Japanese medaka (Oryzias latipes).
复制标题
神经肽Y受体Y2（npy2r）缺乏减少日本青鳉（Oryzias latipes）的焦虑和增加食物摄入量。
DOI：
10.3389/fcell.2023.1273006
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发表时间：
2023
影响因子：
5.5
通讯作者：
Liang, Xu-Fang
中科院分区：
文献类型：
--
作者：
Lu, Ke;Jia, Xiaodan;Wu, Jiaqi;Wang, Qiuling;Liang, Xu-Fang
Neuropeptide Y receptor Y2 (npy2r) is an important receptor gene involved in anxiety and feeding regulation in mammals. Since NPY receptors have different receptor gene deletions in mammals and teleost fish, it is not clear whether npy2r has the similar function in fish as in mammals. In this study, we used the CRISPR/Cas9 system to establish npy2r-deficient medaka (Oryzias latipes). Unexpectedly, the deletion of npy2r resulted in the npy2r +/− medaka were all-male, therefore, npy2r homozygous mutant lines could not be established. The deletion of npy2r increased the food intake in medaka, and the expression levels of appetite stimulating genes (agrp, npy) increased significantly, while the expression levels of anorexia factors (cck, pomc) decreased significantly. Moreover, the absence of npy2r significantly increased the total length and body weight of medaka. The mirror test and open field test showed that npy2r +/− medaka improved sociability and reduced anxiety-like behavior, qRT-PCR analysis showed that the expression levels of anxiety related genes (th1, th2, gr1, gr2, and mr) in npy2r +/− medaka were significantly decreased. So far, this is the first npy2r gene knockout model established in fish and demonstrates that npy2r plays an important role in the regulation of reproduction, feeding and anxiety in fish.
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影响因子：
1.9
作者：
Otsuka, Airi;Shimomura, Yuki;Kagawa, Nao
通讯作者：
Kagawa, Nao
影响因子：
3
作者：
Patil, Jawahar G.;Hinze, Susan J.
通讯作者：
Hinze, Susan J.
影响因子：
64.8
作者：
Hansel, DE;Eipper, BA;Ronnett, GV
通讯作者：
Ronnett, GV
影响因子：
4.6
作者：
Lucon-Xiccato, Tyrone;Loosli, Felix;Conti, Francesca;Foulkes, Nicholas S.;Bertolucci, Cristiano
通讯作者：
Bertolucci, Cristiano
影响因子：
--
作者：
Cerdá-Reverter, JM;Martínez-Rodríguez, G;Larhammar, D
通讯作者：
Larhammar, D