Nitrosospira cluster 3 lineage of AOB and nirK of Rhizobiales respectively dominated N2O emissions from nitrification and denitrification in organic and chemical N fertilizer treated soils
Nitrosospira cluster 3 lineage of AOB and nirK of Rhizobiales respectively dominated N2O emissions from nitrification and denitrification in organic and chemical N fertilizer treated soils
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根瘤菌AOB和nirK的亚硝化螺菌簇3谱系分别主导有机和化学氮肥处理土壤中硝化和反硝化作用的N2O排放
DOI：
10.1016/j.ecolind.2021.107722
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发表时间：
2021-08
影响因子：
6.9
通讯作者：
Zhuge Yuping
中科院分区：
文献类型：
--
作者：
Chen Manman;Pan Hong;Sun Mingjie;He Wei;Wei Meng;Lou Yanhong;Wang Hui;Yang Quangang;Feng Haojie;Zhuge Yuping
农业土壤由于氮（N）肥料的输入和牲畜粪便的排泄而被称为一氧化二氮（N2O）源。然而，很少有研究探讨N2O微生物排放途径及其相关功能团的长期土壤施肥制度的响应。研究了38 a长期定位施肥处理（不施肥对照、无机氮肥、有机肥、无机氮肥配施有机肥）对土壤硝化反硝化过程中N2O生成量以及硝化细菌和异养细菌数量的影响。采用乙炔抑制法确定了N2O排放的微生物途径，并利用分子生物学技术（克隆、高通量测序和定量PCR）研究了硝化菌和硝化菌的反应。施用有机肥显著增加了硝化作用产生的N2O排放量，增加了典型氨氧化菌和完全氨氧化菌（分支B）的丰度，但明显降低了分支A的丰度。结合实验结果，硝化作用产生的N2O含量与细菌固氮菌的丰度呈显著正相关。因此，我们认为施用有机肥刺激了硝化作用产生N2O，硝化作用主要由AOB的Nitrosospiracluster 3谱系控制。与此相反，反硝化作用产生的N2O的量略有刺激化学氮肥，相比，有机施肥。反硝化K型反硝化基因丰度与反硝化产生的N2O排放量之间存在较强的正相关关系。结果表明，单施氮肥可明显促进以非K系根瘤菌为优势菌的反硝化过程中N2O的释放，而化肥和有机肥则分别促进以AOB的Nitrosospiracluster 3和Rhizobiales的非K系根瘤菌为优势菌的反硝化过程和硝化过程中N2O的释放。
Agricultural soils are known as nitrous oxide (N2O) sources due to the input of nitrogen (N) fertilizer and livestock faecal excretion. However, few studies have explored the responses of N2O microbial emission pathways and their related functional guilds to long-term soil fertilization regimes. This study examined the effects of four 38-year long-term fertilization treatments (control with no-fertilizer (CK); inorganic N fertilizer (N); manure (M); inorganic N fertilizer with manure (MN)) on the N2O levels generated from nitrification and denitrification, as well as on the nitrifiers and heterotrophic denitrifiers. The acetylene inhibition method was used to determine the microbial pathways of the N2O emissions and molecular biological techniques (cloning, high-throughput sequencing and quantitative PCR) were performed to investigate the nitrifier and denitrifier responses. The organic fertilization demonstrably increased the N2O emissions from nitrification and the abundance of canonical ammonia oxidizers and complete ammonia oxidizers (clade B), but distinctly decreased the abundance of clade A. Combined with the results that strong and positive relationships were detected between the abundance of bacterialamoAgenes and N2O content produced from nitrification. We proposed that organic fertilizer applications stimulated the generation of N2O from nitrification dominated by theNitrosospiracluster 3 lineage of AOB. In contrast, the amount of N2O produced by denitrification was appreciably stimulated by chemical N fertilization, compared to organic fertilization. Strong and positive relationships were detected between thenirK-type denitrifying gene abundances and the N2O emissions generated from denitrification. This suggested that single N fertilizer additions appreciably stimulated N2O production from denitrification dominated by thenirKlineage of theRhizobiales.These results indicated that chemical N and organic fertilizers stimulated N2O emissions from denitrification and nitrification, respectively, which were dominated by theNitrosospiracluster 3 lineage of AOB and thenirKlineage ofRhizobiales, respectively.
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影响因子：
5.2
作者：
Pjevac P;Schauberger C;Poghosyan L;Herbold CW;van Kessel MAHJ;Daebeler A;Steinberger M;Jetten MSM;Lücker S;Wagner M;Daims H
通讯作者：
Daims H
影响因子：
2.9
作者：
Akiyama, H;McTaggart, IP;Scott, A
通讯作者：
Scott, A
影响因子：
4.2
作者：
Offre, Pierre;Prosser, James I.;Nicol, Graeme W.
通讯作者：
Nicol, Graeme W.
影响因子：
64.8
作者：
van Kessel MA;Speth DR;Albertsen M;Nielsen PH;Op den Camp HJ;Kartal B;Jetten MS;Lücker S
通讯作者：
Lücker S
影响因子：
6.5
作者：
Pan, Hong;Xie, Kexin;Li, Yong
通讯作者：
Li, Yong