Silk scaffolds with tunable mechanical capability for cell differentiation.
Silk scaffolds with tunable mechanical capability for cell differentiation.
复制标题
具有可调机械能力的细胞分化能力的真丝支架。
DOI：
10.1016/j.actbio.2015.04.004
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发表时间：
2015-07
影响因子：
9.7
通讯作者：
Lu, Qiang
中科院分区：
文献类型：
--
作者：
Bai, Shumeng;Han, Hongyan;Huang, Xiaowei;Xu, Weian;Kaplan, David L.;Zhu, Hesun;Lu, Qiang
家蚕丝素蛋白是一种很有前途的组织再生生物材料，通常被认为是一种主动调节细胞分化的惰性材料，因为丝素蛋白的初级序列中几乎没有特定的细胞信号肽结构域，而且由于加工过程中形成的结晶成分，通常具有较高的机械性能。在本研究中，通过丝素纳米纤维辅助冷冻干燥过程，制备了具有纳米结构和可调硬度的丝素蛋白多孔3D支架。在丝素溶液中加入高β-Sheet含量的丝素纳米纤维来调节丝素的自组装，并在冻干后直接诱导水不溶支架的形成。与以前报道的丝素蛋白支架形成过程不同，这些新支架具有更低的总β-Sheet含量和更柔软的机械性能，从而改善了细胞兼容性。支架的硬度可以进一步调整，以匹配软组织的力学性能，从而导致大鼠骨髓间充质干细胞分别向肌源性细胞和内皮细胞分化的结果不同。因此，这些丝素蛋白支架因其机械特性而调节细胞分化结果。
Bombyx mori silk fibroin is a promising biomaterial for tissue regeneration and is usually considered an “inert” material with respect to actively regulating cell differentiation due to few specific cell signaling peptide domains in the primary sequence and the generally stiffer mechanical properties due to crystalline content formed in processing. In the present study, silk fibroin porous 3D scaffolds with nanostructures and tunable stiffness were generated via a silk fibroin nanofiber-assisted lyophilization process. The silk fibroin nanofibers with high β-sheet content were added into the silk fibroin solutions to modulate the self-assembly, and to directly induce water-insoluble scaffold formation after lyophilization. Unlike previously reported silk fibroin scaffold formation processes, these new scaffolds had lower overall β-sheet content and softer mechanical properties for improved cell compatibility. The scaffold stiffness could be further tuned to match soft tissue mechanical properties, which resulted in different differentiation outcomes with rat bone marrow-derived mesenchymal stem cells towards myogenic and endothelial cells, respectively. Therefore, these silk fibroin scaffolds regulate cell differentiation outcomes due to their mechanical features.
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影响因子：
14
作者：
Li, Andrew;Hokugo, Akishige;Yalom, Anisa;Berns, Eric J.;Stephanopoulos, Nicholas;McClendon, Mark T.;Segovia, Luis A.;Spigelman, Igor;Stupp, Samuel I.;Jarrahy, Reza
通讯作者：
Jarrahy, Reza
可逆的水凝胶丝丝系统，具有高β片含量。
影响因子：
6.2
作者：
Bai, Shumeng;Zhang, Xiuli;Lu, Qiang;Sheng, Weiqin;Liu, Lijie;Dong, Boju;Kaplan, David L.;Zhu, Hesun
通讯作者：
Zhu, Hesun
影响因子：
19
作者：
Jiang, Chaoyang;Wang, Xianyan;Tsukruk, Vladimir V.
通讯作者：
Tsukruk, Vladimir V.
影响因子：
56.9
作者：
Cukierman, E;Pankov, R;Yamada, KM
通讯作者：
Yamada, KM
影响因子：
9.7
作者：
Dunne, Lina W.;Iyyanki, Tejaswi;Hubenak, Justin;Mathur, Anshu B.
通讯作者：
Mathur, Anshu B.