Distribution of beta1 integrin during development of chick tarsometatarsal skin in vivo and in vitro.
Distribution of beta1 integrin during development of chick tarsometatarsal skin in vivo and in vitro.
复制标题
体内外鸡跗跖骨皮肤发育过程中β1整合素的分布。
DOI：
10.1177/44.5.8626999
复制
发表时间：
1996
期刊：
影响因子：
--
通讯作者：
H. Hirando
中科院分区：
文献类型：
--
作者：
Y. Akimoto;A. Obinata;H. Endo;H. Hirando
为探讨β1整合素在鸡跖底皮肤发育中的作用，采用免疫组织化学方法和光镜、电子显微镜对β1整合素在体内和体外的定位进行了研究。早期(第5、9、13天)未分化表皮的整个细胞表面均有β1整合素的存在。在表皮角化和层化过程中，β1整合素的定位发生了显著的变化，即从第13天到第17天，β1整合素在浅层和中间细胞层的表达减少。体内17天后，当表皮角化完成时，β1整合素的分布局限于表皮的基底层，呈细胞周围分布。在检查的所有阶段，真皮中的成纤维细胞也被染色。免疫电子显微镜显示，β1整合素定位于角质形成细胞和真皮成纤维细胞的质膜上。在体外分别用氢化可的松或视黄醇诱导角质化(分化)或粘膜化生(转分化)的发育中皮肤的培养中，可以重现体内发生的β1整合素定位的变化。抗β1整合素的单抗在体外可引起表皮角化过程和基底膜结构的显著变化，即抑制角化和基底膜从表皮基面脱落。这些结果表明，β1整合素在细胞-细胞和细胞-细胞外基质相互作用中起重要作用，而细胞-细胞和细胞-细胞外基质相互作用对足底皮肤的表皮发育起重要作用。
To determine the role of beta1 integrin in chick tarsometatarsal skin development, we examined the localization of the beta1 integrin immunohistochemically in vivo and in vitro by light and electron microscopy. Beta1 integrin was present over the entire cell surface of undifferentiated epidermis at early stages (Days 5, 9, 13). Marked changes in the localization of beta1 integrin occurred during epidermal keratinization and stratification, i.e., expression of beta1 integrin decreased in the superficial and intermediate cell layers from Day 13 to Day 17. After 17 days in vivo, when keratinization of the epidermis was completed, the distribution of beta1 integrin was confined to the basal layer of the epidermis in a pericellular distribution. During all stages examined, fibroblasts in the dermis were also stained. Immunoelectron microscopic study revealed that beta1 integrin was located on the plasma membrane of keratinocytes and dermal fibroblasts. The change in beta1 integrin localization that occurred in vivo could be reproduced in cultures of developing skin in which keratinization (differentiation) or mucous metaplasia (transdifferentiation) had been induced in vitro by hydrocortisone or retinol treatment, respectively. A monoclonal antibody against beta1 integrin caused striking changes in the epidermal keratinization process and in the basement membrane structure in vitro, i.e., inhibition of keratinization and detachment of the basement membrane from the basal surface of the epidermis. These results indicate that beta1 integrin plays an important role in cell-cell and cell-extracellular matrix interactions, which are important for epidermal development of the tarsometatarsal skin.
影响因子：
2.7
作者：
BRONNERFRASER, M
通讯作者：
BRONNERFRASER, M
人体皮肤中纤连蛋白、胶原蛋白和层粘连蛋白整合素受体的定位。
DOI：
10.1172/jci114379
发表时间：
1989
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Peltonen,J;Larjava,H;Jaakkola,S;Gralnick,H;Akiyama,SK;Yamada,SS;Yamada,KM;Uitto,J
通讯作者：
Uitto,J