Identification of Autographa californica Nucleopolyhedrovirus ac93 as a Core Gene and Its Requirement for Intranuclear Microvesicle Formation and Nuclear Egress of Nucleocapsids
Identification of Autographa californica Nucleopolyhedrovirus ac93 as a Core Gene and Its Requirement for Intranuclear Microvesicle Formation and Nuclear Egress of Nucleocapsids
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苜蓿银纹夜蛾核多角体病毒ac93核心基因的鉴定及其核内微泡形成和核衣壳出核的要求
DOI：
10.1128/jvi.05275-11
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发表时间：
2011-08
影响因子：
5.4
通讯作者：
Pang, Yi
中科院分区：
文献类型：
--
作者：
Yuan, Meijin;Huang, Zhenqiu;Wei, Denghui;Hu, Zhaoyang;Yang, Kai;Pang, Yi
摘要 苜蓿银纹夜蛾核型多角体病毒（AcMNPV）开放阅读框93（ac93）是一个高度保守但尚未明确特征的基因，除了黑须库蚊核型多角体病毒外，在所有已测序的杆状病毒基因组中均有发现。在本报告中，利用生物信息学分析……
ABSTRACT Autographa californica nucleopolyhedrovirus (AcMNPV) orf93 (ac93) is a highly conserved uncharacterized gene that is found in all of the sequenced baculovirus genomes except for Culex nigripalpus NPV. In this report, using bioinformatics analyses, ac93 and odv-e25 (ac94) were identified as baculovirus core genes and thus p33-ac93-odv-e25 represent a cluster of core genes. To investigate the role of ac93 in the baculovirus life cycle, an ac93 knockout AcMNPV bacmid was constructed via homologous recombination in Escherichia coli. Fluorescence and light microscopy showed that the AcMNPV ac93 knockout did not spread by infection, and titration assays confirmed a defect in budded virus (BV) production. However, deletion of ac93 did not affect viral DNA replication. Electron microscopy indicated that ac93 was required for the egress of nucleocapsids from the nucleus and the formation of intranuclear microvesicles, which are precursor structures of occlusion-derived virus (ODV) envelopes. Immunofluorescence analyses showed that Ac93 was concentrated toward the cytoplasmic membrane in the cytoplasm and in the nuclear ring zone in the nucleus. Western blot analyses showed that Ac93 was associated with both nucleocapsid and envelope fractions of BV, but only the nucleocapsid fraction of ODV. Our results suggest that ac93, although not previously recognized as a core gene, is one that plays an essential role in the formation of the ODV envelope and the egress of nucleocapsids from the nucleus.
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影响因子：
--
作者：
Slack J;Arif BM
通讯作者：
Arif BM
DOI：
--
发表时间：
2007
期刊：
Biotechnology(faisalabad)
影响因子：
--
作者：
P. Yi
通讯作者：
P. Yi
影响因子：
5.4
作者：
Gonnella, R;Farina, A;Faggioni, A
通讯作者：
Faggioni, A
DOI：
10.1073/pnas.0701757104
发表时间：
2007-04-24
影响因子：
11.1
作者：
Klupp, Barbara G.;Granzow, Harald;Mettenleiter, Thomas C.
通讯作者：
Mettenleiter, Thomas C.
影响因子：
3.7
作者：
Long, C. M.;Rohrmann, G. F.;Merrill, G. F.
通讯作者：
Merrill, G. F.