Dynamics of shape recovery by stored red blood cells during washing at the single cell level.
Dynamics of shape recovery by stored red blood cells during washing at the single cell level.
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DOI：
10.1111/trf.15979
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发表时间：
2020-10
期刊：
影响因子：
2.9
通讯作者：
Shevkoplyas, Sergey S.
中科院分区：
文献类型：
--
作者：
Lu, Madeleine;Shevkoplyas, Sergey S.
低温储存将红细胞（RBC）从光滑的双凹面盘状细胞转变为越来越球形的针状棘红细胞，并最终转变为易碎的球形红细胞。单个细胞以不同的速率经历这种转化，在每个储存血液单位中的棘红细胞增多的所有阶段产生RBC的异质混合物。在这里，我们研究了洗涤（已知对RBC特性有积极影响）如何在单细胞水平上改变单个RBC的形态。我们追踪了在盐水（n = 1095）、1%人血清白蛋白（1%HSA）溶液（n = 999）和自体储存上清液（对照，n = 776）中洗涤期间限制在微流体威尔斯孔阵列中的单个RBC（n = 2870;从6个4-6周龄RBC单位中抽取）的形状变化。形状恢复通过针状体的消失进行，随后是RBC轮廓的逐渐平滑，其中大部分变化发生在暴露于洗涤溶液的最初10分钟内。约57%的所有棘红细胞恢复至少一个形态分类时，在1%HSA（36%的生理盐水）洗涤，与3%的细胞在后期棘红细胞完全恢复其盘状形状。约1/3的球形细胞在任一洗涤液中裂解。在其自体储存上清液中洗涤的细胞在洗涤期间继续恶化。我们的研究结果表明，在洗涤过程中用洗涤液替换储存上清液诱导红细胞在棘红细胞增多的所有阶段的实际形状恢复，除了球形红细胞进行裂解。
Hypothermic storage transforms red blood cells (RBC) from smooth biconcave discocytes into increasingly spherical spiculated echinocytes and, ultimately, fragile spherocytes. Individual cells undergo this transformation at different rates, producing a heterogeneous mixture of RBCs at all stages of echinocytosis in each unit of stored blood. Here we investigated how washing (known to positively affect RBC properties) changes morphology of individual RBCs at the single-cell level. We tracked the change in shape of individual RBCs (n = 2870; drawn from six 4-6-week old RBC units) that were confined in an array of microfluidic wells during washing in saline (n = 1095), 1% human serum albumin (1% HSA) solution (n = 999), and the autologous storage supernatant (control, n = 776). Shape recovery proceeded through the disappearance of spicules followed by the progressive smoothening of the RBC contour, with the majority of changes occurring within the initial 10 min of being exposed to the washing solution. About 57% of all echinocytes recovered by at least one morphological class when washed in 1% HSA (36% for normal saline), with 3% of cells in late-stage echinocytosis restoring their discoid shape completely. About 1/3 of all spherocytic cells lysed in either washing solution. Cells washed in their autologous storage supernatant continued to deteriorate during washing. Our findings suggest that the replacement of storage supernatant with a washing solution during washing induces actual shape recovery for RBCs in all stages of echinocytosis, except for spherocytes that undergo lysis instead.
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影响因子：
2.9
作者：
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DOI：
10.1073/pnas.0708160104
发表时间：
2007-10-23
影响因子：
11.1
作者：
Bennett-Guerrero, Elliott;Veldman, Tim H.;McMahon, Timothy J.
通讯作者：
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