Changes in Band 3 oligomeric state precede cell membrane phospholipid loss during blood bank storage of red blood cells.
Changes in Band 3 oligomeric state precede cell membrane phospholipid loss during blood bank storage of red blood cells.
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DOI：
10.1111/j.1537-2995.2009.02133.x
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发表时间：
2009-07
期刊：
影响因子：
2.9
通讯作者：
Thomas DD
中科院分区：
文献类型：
--
作者：
Karon BS;Hoyer JD;Stubbs JR;Thomas DD
囊泡形式的脂质损失有助于红细胞（RBC）储存损伤，并且这种脂质损失与膜蛋白功能的变化相关。灵敏的光谱技术用于测量红细胞储存期间带3寡聚状态的变化，与代谢变化和磷脂损失相比。本研究的目的是确定红细胞储存过程中膜蛋白大分子组织的变化是否发生在脂质损失之前、同时或之后。从正常志愿者中采集5个RBC单位，并储存在标准血库条件下，通过多种测定测量代谢变化和脂质损失。通过时间分辨磷光各向异性和曙红-5-马来酰亚胺标记的红细胞血影的荧光共振能量转移来评估带3寡聚状态。血液冷藏过程中细胞外pH值迅速下降，细胞外钾迅速升高。RBC膜上的条带3在储存期早期和RBC膜磷脂可检测损失之前表现出从小寡聚体到大寡聚体的转变。带3，这是通过血影蛋白和锚蛋白拴在细胞骨架网络的固定化部分，没有改变在冷藏过程中。我们的研究结果表明，红细胞膜蛋白的大分子组织的变化发生在存储的早期，这些变化可能会导致磷脂损失，不可逆的形态变化，和红细胞存储过程中的功能丧失。
Lipid loss in the form of vesicles contributes to the red blood cell (RBC) storage lesion, and this loss of lipid is correlated with changes in membrane protein function. Sensitive spectroscopic techniques were used to measure changes in Band 3 oligomeric state during storage of RBCs, compared to metabolic changes and phospholipid loss. The aim of the study was to determine whether changes in the macromolecular organization of membrane proteins occur before, coincident with, or after lipid loss during RBC storage. Five RBC units were collected from normal volunteers and stored under standard blood bank conditions, and both metabolic changes and lipid loss were measured by multiple assays. Band 3 oligomeric state was assessed by time-resolved phosphorescence anisotropy and fluorescence resonance energy transfer of eosin-5-maleimide–labeled RBC ghosts. Extracellular pH decreased and extracellular potassium increased rapidly during cold storage of blood. Band 3 on the RBC membrane exhibited a shift from small to large oligomers early in the storage period and before detectable loss of phospholipid from the RBC membrane. The immobilized fraction of Band 3, that which is tethered to the cytoskeletal network via spectrin and ankyrin, did not change during cold storage. Our results demonstrate that changes in the macromolecular organization of membrane proteins on the RBC occur early in storage, and these changes may induce phospholipid loss, irreversible morphologic changes, and loss of function during RBC storage.
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影响因子：
5.8
作者：
ELLMAN, GL;COURTNEY, KD;FEATHERSTONE, RM
通讯作者：
FEATHERSTONE, RM
影响因子：
2.9
作者：
Bosman, Giel J. C. G. M.;Lasonder, Edwin;De Grip, Willem J.
通讯作者：
De Grip, Willem J.
影响因子：
2.9
作者：
Cho, MR;Eber, SW;Golan, DE
通讯作者：
Golan, DE
影响因子：
2.9
作者：
COBB, CE;BETH, AH
通讯作者：
BETH, AH
DOI：
10.3109/00365518109097442
发表时间：
1981-01-01
影响因子：
2.1
作者：
ROELOFSEN, B;VAN MEER, G;DENKAMP, JAFO
通讯作者：
DENKAMP, JAFO