Drosophila chibby is required for basal body formation and ciliogenesis but not for Wg signaling.
Drosophila chibby is required for basal body formation and ciliogenesis but not for Wg signaling.
复制标题
果蝇 chibby 是基体形成和纤毛发生所必需的，但不是 Wg 信号传导所必需的。
DOI：
10.1083/jcb.201109148
复制
发表时间：
2012-04-16
期刊：
影响因子：
--
通讯作者：
Durand B
中科院分区：
文献类型：
--
作者：
Enjolras C;Thomas J;Chhin B;Cortier E;Duteyrat JL;Soulavie F;Kernan MJ;Laurençon A;Durand B
与脊椎动物CBY在WNT信号传导中起作用相反，果蝇CBY对正常的基体结构和功能是必需的，但对Wg信号传导是必需的。中心粒向基体的转化是纤毛发生的一个复杂过程，包括向中心粒中添加特定的蛋白质。CHIBBY（CBY）是一种卷曲螺旋结构域蛋白，首先被描述为与β-连环蛋白相互作用并参与Wg-Int（WNT）信号传导。我们发现，在黑腹果蝇中，CBY只在需要功能性基底体的细胞中表达，即，感觉神经元和雄性生殖细胞。CBY与这两种细胞类型的基体过渡区（TZ）有关。cby的失活导致感觉传导和精子发生的缺陷。CBY的损失导致改变纤毛贩运成神经元纤毛，不规则沉积的精母细胞基体上的蛋白质，因此，扭曲的轴丝组装。重要的是，cby 1/1果蝇没有表现出无翅信号缺陷。因此，CBY对果蝇正常的基体结构和功能至关重要，可能通过对TZ的影响。CBY在脊椎动物WNT信号转导中的功能在脊椎动物进化过程中获得或在果蝇中丢失。
In contrast to vertebrate CBY, which functions in WNT signaling, Drosophila CBY is essential for normal basal body structure and function but dispensable for Wg signaling. Centriole-to–basal body conversion, a complex process essential for ciliogenesis, involves the progressive addition of specific proteins to centrioles. CHIBBY (CBY) is a coiled-coil domain protein first described as interacting with β-catenin and involved in Wg-Int (WNT) signaling. We found that, in Drosophila melanogaster, CBY was exclusively expressed in cells that require functional basal bodies, i.e., sensory neurons and male germ cells. CBY was associated with the basal body transition zone (TZ) in these two cell types. Inactivation of cby led to defects in sensory transduction and in spermatogenesis. Loss of CBY resulted in altered ciliary trafficking into neuronal cilia, irregular deposition of proteins on spermatocyte basal bodies, and, consequently, distorted axonemal assembly. Importantly, cby1/1 flies did not show Wingless signaling defects. Hence, CBY is essential for normal basal body structure and function in Drosophila, potentially through effects on the TZ. The function of CBY in WNT signaling in vertebrates has either been acquired during vertebrate evolution or lost in Drosophila.
登录
查看更多内容
影响因子：
4
作者：
El Zein, Loubna;Ait-Lounis, Aouatef;Durand, Benedicte
通讯作者：
Durand, Benedicte
影响因子：
3.5
作者：
Jiang, Si-Tse;Chiou, Yuan-Yow;Li, Hung
通讯作者：
Li, Hung
影响因子：
64.5
作者：
JARMAN, AP;GRAU, Y;JAN, YN
通讯作者：
JAN, YN
影响因子：
9.2
作者：
Bettencourt-Dias, M;Rodrigues-Martins, A;Glover, DM
通讯作者：
Glover, DM
影响因子：
4.6
作者：
Dubruille, R;Laurençon, A;Durand, B
通讯作者：
Durand, B