Host cells subdivide nutrient niches into discrete biogeographical microhabitats for gut microbes.
Host cells subdivide nutrient niches into discrete biogeographical microhabitats for gut microbes.
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DOI：
10.1016/j.chom.2022.04.012
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发表时间：
2022-06-08
影响因子：
30.3
通讯作者：
Baumler, Andreas J.
中科院分区：
文献类型：
--
作者：
Liou, Megan J.;Miller, Brittany M.;Litvak, Yael;Nguyen, Henry;Natwick, Dean E.;Savage, Hannah P.;Rixon, Jordan A.;Mahan, Scott P.;Hiyoshi, Hirotaka;Rogers, Andrew W. L.;Velazquez, Eric M.;Butler, Brian P.;Collins, Sean R.;McSorley, Stephen J.;Harshey, Rasika M.;Byndloss, Mariana X.;Simon, Scott I.;Baumler, Andreas J.
微生物群组成的变化与许多人类疾病有关，但控制菌株丰度的因素仍然定义不清。我们发现，大肠杆菌菌株和致病性沙门氏菌肠血清型鼠伤寒分离株都利用硝酸盐的肠道生长，但每个访问这个资源在一个不同的地理生态位。共栖E.大肠杆菌利用上皮来源的硝酸盐，而S.鼠伤寒沙门氏菌来源于吞噬细胞浸润。令人惊讶的是，无毒S.鼠伤寒沙门氏菌不能利用上皮来源的硝酸盐，因为它的趋化性受体McpB和McpC将病原体从E.杆菌与之相反，E. coli侵入S.鼠伤寒毒力因子，并通过竞争硝酸盐赋予定植抗性。因此，营养生态位并不仅仅由关键资源来定义，而是可以在宿主内进一步细分为不同的微生境，从而为不同的细菌物种产生新的生态位机会。Liou等发现致病性沙门氏菌和大肠杆菌。大肠杆菌在不同的空间微生境中获得关键资源硝酸盐，这些微生境由不同的宿主细胞类型产生。E.大肠杆菌入侵沙门氏菌占据的空间微生境有助于定植抗性。
Changes in the microbiota composition are associated with many human diseases, but factors governing strain abundance remain poorly defined. We show that a commensal Escherichia coli strain and a pathogenic Salmonella enterica serovar Typhimurium isolate both utilized nitrate for intestinal growth, but each accessed this resource in a distinct biogeographical niche. Commensal E. coli utilized epithelial-derived nitrate, whereas nitrate in the niche occupied by S. Typhimurium was derived from phagocytic infiltrates. Surprisingly, avirulent S. Typhimurium was unable to utilize epithelial-derived nitrate, because its chemotaxis receptors McpB and McpC excluded the pathogen from the niche occupied by E. coli. In contrast, E. coli invaded the niche constructed by S. Typhimurium virulence factors and conferred colonization resistance by competing for nitrate. Thus, nutrient-niches are not defined solely by critical resources but can be further subdivided biogeographically within the host into distinct microhabitats, thereby generating new niche opportunities for distinct bacterial species. Liou et al. find that pathogenic Salmonella and commensal E. coli obtain a critical resource, nitrate, in distinct spatial microhabitats in the gut lumen, which are generated by different host cell types. The ability of E. coli to invade the spatial microhabitat occupied by Salmonella contributes to colonization resistance.
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DOI：
10.1126/science.aam9949
发表时间：
2017-08-11
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Byndloss MX;Olsan EE;Rivera-Chávez F;Tiffany CR;Cevallos SA;Lokken KL;Torres TP;Byndloss AJ;Faber F;Gao Y;Litvak Y;Lopez CA;Xu G;Napoli E;Giulivi C;Tsolis RM;Revzin A;Lebrilla CB;Bäumler AJ
通讯作者：
Bäumler AJ
影响因子：
2.8
作者：
Greer-Phillips, SE;Alexandre, G;Zhulin, IB
通讯作者：
Zhulin, IB
影响因子：
1.2
作者：
Ahmadi, Shokouh;Wang, Shaohua;Yadav, Hariom
通讯作者：
Yadav, Hariom
影响因子：
30.3
作者：
Eberl, Claudia;Weiss, Anna S.;Stecher, Barbel
通讯作者：
Stecher, Barbel
影响因子：
3.7
作者：
Loetscher Y;Wieser A;Lengefeld J;Kaiser P;Schubert S;Heikenwalder M;Hardt WD;Stecher B
通讯作者：
Stecher B