Wet nanoscale imaging and testing of polymersomes.
Wet nanoscale imaging and testing of polymersomes.
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DOI：
10.1002/smll.201100511
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发表时间：
2011-07-18
期刊：
影响因子：
13.3
通讯作者：
Engler, Adam J.
中科院分区：
文献类型：
--
作者：
Battaglia, Giuseppe;LoPresti, Caterina;Massignani, Marzia;Warren, Nicholas J.;Madsen, Jeppe;Forster, Simon;Vasilev, Cvetilin;Hobbs, Jamie K.;Armes, Steven P.;Chirasatitsin, Somoyot;Engler, Adam J.
Giuseppe Battaglia，* Caterina LoPresti，Marzia Massignani，Nicholas J. Warren，Jeppe Madsen，Simon Forster，Cvetilin Vasilev，Jamie K. Hobbs，Steven P. Armes，Somoyot Chirasatis，和Adam J. Engler所述的方法，该方法既用于成像，也用于评估机械、电气和表面性质。[7]然而，AFM在液体中成像时需要颗粒被固定。通过共价连接[8]和非共价键合（例如，利用生物素/链霉亲和素复合[9]或库仑相互作用）实现了颗粒和聚合物囊泡的固定。[10]在这里，我们提出了两种策略，用于用生物素轻松官能化聚合物囊泡，并随后将其固定在链霉亲和素包被的表面上。这种通用方法使力谱映射（FSM）[11]能够在水性环境中对中性聚合物囊泡进行成像。如图1A所示，第一种方法通过向两亲性嵌段共聚物混合物中加入市售生物素-1，2-二硬脂酰基-sn-甘油基-3-磷酸乙醇胺-N-[生物素基（聚乙二醇）-2000]（生物素-PEG-DSPE）来进行超分子官能化。第二种方法是用生物素对嵌段共聚物进行化学官能化。在这两种情况下，都使用了聚（（2-甲基丙烯酰氧）乙基磷酰胆碱）-嵌段-聚（甲基丙烯酸2-（二异丙基氨基）乙酯）（PMPC 25-PDPA 70）二嵌段共聚物。PMPC-PDPA聚合物囊泡是pH敏感的，并且已经报道有效地进入细胞并在其胞质溶胶内递送其货物，而没有毒性。
Giuseppe Battaglia,* Caterina LoPresti, Marzia Massignani, Nicholas J. Warren, Jeppe Madsen, Simon Forster, Cvetilin Vasilev, Jamie K. Hobbs, Steven P. Armes, Somoyot Chirasatitsin, and Adam J. Engler that has been used for both imaging and also for assessing mechanical, electrical, and surface properties.[7] However, AFM requires particles to be immobilized when imaging in liquid. Immobilization of particles and polymersomes in particular has been achieved by both covalent attachment [8] and noncovalent bonding, eg, by exploiting biotin/streptavidin complexation [9] or Coulombic interactions.[10]Here we present two strategies for the facile functionalization of polymersomes with biotin and their subsequent immobilization on streptavidin-coated surfaces. This generic approach enables force spectroscopy mapping (FSM)[11] to image neutral polymersomes in an aqueous environment. As illustrated in Figure 1A, the first approach employs supramolecular functionalization by the addition of commercially available Biotin-1, 2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[biotinyl (polyethylene glycol)-2000](Biotin–PEG–DSPE) to an amphiphilic block copolymer mixture. The second approach is the chemical functionalization of the block copolymer with biotin. In both cases, a poly ((2-methacryloyloxy) ethyl phosphorylcholine)-block-poly (2-(diisopropylamino) ethyl methacrylate)(PMPC 25–PDPA 70) diblock copolymers were used. PMPC–PDPA polymersomes are pH-sensitive and have been reported to efficiently enter cells and deliver their cargo within their cytosol with no toxic
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影响因子：
17.1
作者：
LoPresti, Caterina;Massignani, Marzia;Battaglia, Giuseppe
通讯作者：
Battaglia, Giuseppe
影响因子：
56.9
作者：
Discher, BM;Won, YY;Hammer, DA
通讯作者：
Hammer, DA
影响因子：
2.7
作者：
Chirasatitsin, Somyot;Engler, Adam J.
通讯作者：
Engler, Adam J.
影响因子：
4.7
作者：
Christian, Natalie A.;Milone, Michael C.;Hammer, Daniel A.
通讯作者：
Hammer, Daniel A.
影响因子：
4.9
作者：
Sauer, M;Haefele, T;Meier, W
通讯作者：
Meier, W