SR alpha promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat
SR alpha promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat
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SR α启动子：一种高效、多功能的哺乳动物cDNA表达系统，由猿猴病毒40早期启动子和人T细胞白血病病毒1型长末端重复序列的R-U5片段组成
DOI：
10.1128/mcb.8.1.466-472.1988
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发表时间：
1988
影响因子：
5.3
通讯作者：
N. Arai
中科院分区：
文献类型：
--
作者：
Y. Takebe;M. Seiki;J. Fujisawa;P. Hoy;K. Yokota;K. Arai;Mitsuaki Yoshida;N. Arai
我们开发了一种新的启动子系统，命名为SR α，它由猿猴病毒40（SV 40）早期启动子和人T细胞白血病病毒1型长末端重复序列的R片段和部分U 5序列（R-U 5 '）组成。R-U 5 '序列仅当以有义方向紧邻SV 40早期启动子下游时刺激氯霉素乙酰转移酶（CAT）基因表达。SR α表达系统在包括成纤维细胞和淋巴样细胞在内的多种细胞类型中比SV 40早期启动子活性高1或2个数量级，并且能够促进各种淋巴因子cDNA的高水平表达。SR α启动子的这些特征被整合到最初由冈山和贝格开发的pcD-cDNA表达克隆载体中。
We developed a novel promoter system, designated SR alpha, which is composed of the simian virus 40 (SV40) early promoter and the R segment and part of the U5 sequence (R-U5') of the long terminal repeat of human T-cell leukemia virus type 1. The R-U5' sequence stimulated chloramphenicol acetyltransferase (CAT) gene expression only when placed immediately downstream of the SV40 early promoter in the sense orientation. The SR alpha expression system was 1 or 2 orders of magnitude more active than the SV40 early promoter in a wide variety of cell types, including fibroblasts and lymphoid cells, and was capable of promoting a high level of expression of various lymphokine cDNAs. These features of the SR alpha promoter were incorporated into the pcD-cDNA expression cloning vector originally developed by Okayama and Berg.
人 T 细胞白血病病毒 II 型长末端重复序列的核苷酸序列分析。
DOI：
10.1073/pnas.81.4.1079
发表时间：
1984
影响因子：
11.1
作者：
Shimotohno,K;Golde,DW;Miwa,M;Sugimura,T;Chen,IS
通讯作者：
Chen,IS
负责人 T 细胞白血病病毒 I 型转录激活的顺式作用序列构成了条件增强子。
DOI：
10.1073/pnas.83.17.6558
发表时间：
1986
影响因子：
11.1
作者：
Paskalis,H;Felber,BK;Pavlakis,GN
通讯作者：
Pavlakis,GN