Laser-induced fluorescence detection of malignant gliomas using fluorescein-labeled serum albumin: Experimental and preliminary clinical results
Laser-induced fluorescence detection of malignant gliomas using fluorescein-labeled serum albumin: Experimental and preliminary clinical results
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使用荧光素标记血清白蛋白激光诱导荧光检测恶性神经胶质瘤：实验和初步临床结果
DOI：
10.1080/01616412.2000.11740705
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发表时间：
2000
影响因子：
1.9
通讯作者：
S. Kunze
中科院分区：
文献类型：
--
作者：
Paul Kremer;Andreas Wunder;H. Sinn;T. Haase;M. Rheinwald;Uwe Zillmann;F. K. Albert;S. Kunze
摘要应用激光诱导荧光检测技术，以5-氨基荧光素-白蛋白为荧光染料，对恶性胶质瘤的肿瘤边缘进行了定位。使用残基标记物将5-氨基荧光素连接至血清白蛋白（= AFlc-SA）作为累积蛋白标记。在C6-胶质瘤模型中，通过用111 In-DTPA标记蛋白缀合物来研究注射的染料的生物分布和药代动力学。静脉注射染料后24小时，用氩激光激活荧光，并在C6神经胶质瘤中进行检查。进行组织学检查以比较荧光染色肿瘤与苏木精/伊红的显微镜边缘。染料注射后24小时的肿瘤摄取比周围脑中高23倍。激光激活下的荧光检查显示了清晰染色和清晰分界的肿瘤。肿瘤的显微边界与荧光完全对应，也表明细胞内肿瘤对染料的吸收。在初步研究中，三名恶性胶质瘤患者在注射AFlc-SA后使用激光诱导荧光检测技术进行手术。在所有患者中，恶性胶质瘤的边界在手术过程中被AFlc-SA清楚地染色。使用白蛋白缀合物AFlc-SA的激光诱导荧光成像可能是一种有前途的方法，用于描绘仅在手术显微镜下难以检测的肿瘤边缘。[Neurol Res 2000; 22：481-489]
Abstract To delineate the tumor margins of malignant gliomas laser-induced fluorescence detection technique was applied using 5-aminofluoresceln-albumin as the fluorescent dye. The 5-aminofluorescein was linked to serum albumin (= AFlc-SA) as a cumulative protein label using residualizing markers. In a C6-glioma model the biodistribution and pharmacokinetics of the injected dye were investigated by labeling the protein conjugate with 111 In-DTPA. Twenty-four hours after Intravenous Injection of the dye, fluorescence was activated by an argon laser and inspected in the C6-gliomas. Histological examinations were performed to compare the microscopic margins of the fluorescence-stained tumors with hematoxylin/eosin. The tumor uptake 24 h after dye injection was 23-fold higher than in the surrounding brain. Fluorescence inspection under laser activation demonstrated clearly stained and sharply demarcated tumors. The microscopic borders of the tumors corresponded exactly with the fluorescence, also demonstrating intracellular tumor uptake of the dye. In a preliminary study, three patients with malignant gliomas were operated using laser-induced fluorescence detection technique after injection of AFlc-SA. In all patients, the borders of the malignant gliomas were clearly stained by AFlc-SA during surgery. Laser-induced fluorescence imaging using the albumin conjugate AFlc-SA may be a promising method for delineating tumor margins which are hard to detect under the operating microscope alone. [Neurol Res 2000; 22: 481-489]
一种放射性碘标记的细胞内捕获配体，用于确定体内血浆蛋白降解的位点。
DOI：
10.1042/bj2120791
发表时间：
1983
期刊：
The Biochemical journal
影响因子：
--
作者：
Pittman,RC;Carew,TE;Glass,CK;Green,SR;TaylorJr,CA;Attie,AD
通讯作者：
Attie,AD
影响因子：
4.8
作者：
Haglund, MM;Berger, MS;Hochman, DW
通讯作者：
Hochman, DW
影响因子：
19.7
作者：
C. Zimmer;R. Weissleder;K. Poss;Anna Bogdanova;S. C. Wright;W. S. Enochs
通讯作者：
C. Zimmer;R. Weissleder;K. Poss;Anna Bogdanova;S. C. Wright;W. S. Enochs