The Copy Number Variation of OsMTD1 Regulates Rice Plant Architecture.
The Copy Number Variation of OsMTD1 Regulates Rice Plant Architecture.
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OsMTD1 拷贝数变异调节水稻植株结构
DOI：
10.3389/fpls.2020.620282
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发表时间：
2020
影响因子：
5.6
通讯作者：
Xiao L
中科院分区：
文献类型：
--
作者：
Liu Q;Xu J;Zhu Y;Mo Y;Yao XF;Wang R;Ku W;Huang Z;Xia S;Tong J;Huang C;Su Y;Lin W;Peng K;Liu CM;Xiao L
拷贝数变异（CNV）可通过改变所含基因或调控元件的表达水平而具有表型效应。据信，CNV在控制植物的结构和其他性状方面起着关键作用。然而，CNV对特殊性状的影响在很大程度上仍然未知。在这里，我们报告了一个CNV通过调节分蘖数和叶角参与水稻结构。在水稻（Oryza sativa ssp.日本在水稻分蘖调控基因OsMTD 1附近区域的13，002-bp串联重复序列中发现了一个位点Loc_Os08g34249。对230个水稻品种的进一步调查表明，只有13个粳稻品种发生了重复。表型研究表明，该CNV区域可能有助于分蘖数。此外，OsMTD 1基因不仅影响水稻分蘖数和叶片角度，而且抑制了pri-miR 156 f在CNV区域的转录。有趣的是，这种CNV通过对OsMTD 1和pri-miR 156 f的剂量和位置效应发挥作用。因此，我们的工作鉴定了一个CNV，揭示了其对植株构型影响的分子调控基础，表明该CNV在水稻分子育种中具有重要意义和应用潜力。
Copy number variation (CNV) may have phenotypic effects by altering the expression level of the gene(s) or regulatory element(s) contained. It is believed that CNVs play pivotal roles in controlling plant architecture and other traits in plant. However, the effects of CNV contributing to special traits remain largely unknown. Here we report a CNV involved in rice architecture by modulating tiller number and leaf angle. In the genome of Oryza sativa ssp. japonica cv. Nipponbare, we found a locus Loc_Os08g34249 is derived from a 13,002-bp tandem duplication in the nearby region of OsMTD1, a gene regulating tillering in rice. Further survey of 230 rice cultivars showed that the duplication occurred in only 13 japonica rice cultivars. Phenotypic investigation indicated that this CNV region may contribute to tiller number. Moreover, we revealed that OsMTD1 not only influences rice tiller number and leaf angle, but also represses pri-miR156f transcription in the CNV region. Intriguingly, this CNV performs function through both the dosage and position effects on OsMTD1 and pri-miR156f. Thus, our work identified a CNV and revealed a molecular regulatory basis for its effects on plant architecture, implying this CNV may possess importance and application potential in molecular breeding in rice.
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影响因子：
16.6
作者：
Zhang L;Yu H;Ma B;Liu G;Wang J;Wang J;Gao R;Li J;Liu J;Xu J;Zhang Y;Li Q;Huang X;Xu J;Li J;Qian Q;Han B;He Z;Li J
通讯作者：
Li J
DOI：
10.1016/j.tig.2015.05.010
发表时间：
2015-10
期刊：
Trends in genetics : TIG
影响因子：
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作者：
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通讯作者：
Rudd MK
影响因子：
30.8
作者：
Cong, Bin;Barrero, Luz S.;Tanksley, Steven D.
通讯作者：
Tanksley, Steven D.
影响因子：
56.9
作者：
Sebat, J;Lakshmi, B;Wigler, M
通讯作者：
Wigler, M
影响因子：
30.8
作者：
Lupski, James R.
通讯作者：
Lupski, James R.