Detection of mutated K-ras DNA in urine, plasma, and serum of patients with colorectal carcinoma or adenomatous polyps.
Detection of mutated K-ras DNA in urine, plasma, and serum of patients with colorectal carcinoma or adenomatous polyps.
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DOI：
10.1196/annals.1448.027
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发表时间：
2008-08
影响因子：
5.2
通讯作者：
Block TM
中科院分区：
文献类型：
--
作者：
Su YH;Wang M;Brenner DE;Norton PA;Block TM
我们之前的研究表明，尿液中含有来自循环系统的 DNA，并且这种 DNA 部分源自泌尿道远端的器官部位和肿瘤。为了探索与其他体液相比，尿液中的 DNA 作为用于癌症检测的循环 DNA 来源的潜在用途，检查了血清、血浆和尿液中突变 Kristin-ras (K-ras) DNA 的 DNA 浓度和检测频率。尿液中DNA浓度与血清中相似，但血浆中DNA浓度显着低于尿液或血清（P < 0.05）。当在每次突变检测中使用来自 10 μL 体液的 DNA 时，血清、血浆和尿液中突变 K-ras DNA 的检测频率相当。然而，当使用源自200μL体液的DNA时，尿液中检测到突变K-ras DNA的发生率(95%)显着高于血清(35%)或血浆(40%)(P<0.0005)，这表明血清/血浆中的抑制因子可能比尿液中的抑制因素更具限制性。讨论了尿液作为癌症检测循环 DNA 来源的用途和实用性。
Our previous studies demonstrated that urine contains DNA derived from the circulation and that this DNA originated, in part, from organ sites and tumors distal to the urinary tract. To explore the potential use of DNA from urine as compared to other body fluids as a source for circulating DNA for cancer detection, the DNA concentration and the frequency of detection of mutated Kristin-ras (K-ras) DNA in serum, plasma, and urine were examined. The concentration of DNA in the urine was similar to that in the serum, but the DNA concentration in plasma was significantly lower than in either urine or serum (P < 0.05). When DNA derived from 10 μL of body fluid was used in each mutation assay, the detection frequency of mutated K-ras DNA was comparable among serum, plasma, and urine. However, when DNA derived from 200 μL of body fluid was used, the incidence of detecting mutated K-ras DNA in urine was significant higher (95%) than in either serum (35%) or plasma (40%) (P < 0.0005), suggesting that inhibitory factors in serum/plasma may be more limiting than in urine. The use and practicality of urine as a source of circulating DNA for cancer detection are discussed.
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影响因子：
15.9
作者：
KOFFLER, D;AGNELLO, V;KUNKEL, HG
通讯作者：
KUNKEL, HG
影响因子：
82.9
作者：
Nawroz, H;Koch, W;Sidransky, D
通讯作者：
Sidransky, D
影响因子：
29.4
作者：
Ahlquist, DA;Skoletsky, JE;Shuber, AP
通讯作者：
Shuber, AP
影响因子：
3.5
作者：
SchulteHermann, R;Bursch, W;Mullauer, L
通讯作者：
Mullauer, L
影响因子：
2.2
作者：
NAKAMURA, T;SAKAI, T;NARIYA, S
通讯作者：
NARIYA, S