Maize ZmBES1/BZR1-3 and -9 Transcription Factors Negatively Regulate Drought Tolerance in Transgenic Arabidopsis.
Maize ZmBES1/BZR1-3 and -9 Transcription Factors Negatively Regulate Drought Tolerance in Transgenic Arabidopsis.
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DOI：
10.3390/ijms23116025
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发表时间：
2022-05-27
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
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作者：
BRI 1-EMS抑制子1（BES 1）/油菜素耐药基因1（BZR 1）转录因子在植物生长发育和逆境应答中起着重要作用。然而，对玉米的BES 1/BZR 1的功能知之甚少。本研究从玉米自交系B73中克隆了ZmBES 1/BZR 1 -3和ZmBES 1/BZR 1 -9基因，并通过分析其表达模式、亚细胞定位、转录激活活性以及在拟南芥中的异源表达，对其功能进行了研究。qRT-PCR结果显示，ZmBES 1/BZR 1 -3和ZmBES 1/BZR 1 -9基因主要在根中表达，干旱胁迫使其表达量显著下调。ZmBES 1/BZR 1 -3和ZmBES 1/BZR 1 -9蛋白定位于细胞核中，但作为单体不显示转录激活活性。随后，发现ZmBES 1/BZR 1 -3和ZmBES 1/BZR 1 -9基因在拟南芥中的异源表达分别降低了耐旱性。干旱胁迫下，与对照相比，转基因株系表现出更严重的萎蔫表型，根长缩短，鲜重降低，相对电解质渗漏率（REL）和丙二醛（MDA）含量升高。RNA测序结果表明，ZmBES 1/BZR 1 -3和ZmBES 1/BZR 1 -9转基因拟南芥中差异表达基因（DEG）的表达量分别为70.67%和93.27%。ZmBES 1/BZR 1 -3基因表达系的DEG主要与氧化应激反应和氨基酸代谢过程有关，并在内质网中富集苯丙素类物质的合成和蛋白质加工。而ZmBES 1/BZR 1 -9基因表达系的DEG主要与水分胁迫、胞外刺激和茉莉酸有关，富含苯丙素类化合物的合成和植物激素信号转导。此外，ZmBES 1/BZR 1 -9增加了转基因拟南芥在干旱胁迫下的气孔开度。本研究表明ZmBES 1/BZR 1 -3和ZmBES 1/BZR 1 -9通过不同的途径对转基因拟南芥的耐旱性进行负调控，为深入了解BES 1/BZR 1 s在作物中的功能提供了理论依据。
The BRI1-EMS suppressor 1 (BES1)/brassinazole-resistant 1(BZR1) transcription factors play crucial roles in plant growth, development, and stress response. However, little is known about the function of maize’s BES1/BZR1s. In this study, the ZmBES1/BZR1-3 and ZmBES1/BZR1-9 genes were cloned from maize’s inbred line, B73, and they were functionally evaluated by analyzing their expression pattern, subcellular localization, transcriptional activation activity, as well as their heterologous expression in Arabidopsis, respectively. The results of the qRT-PCR showed that the ZmBES1/BZR1-3 and ZmBES1/BZR1-9 genes were predominantly expressed in the root, and their expression was significantly down-regulated by drought stress. The ZmBES1/BZR1-3 and ZmBES1/BZR1-9 proteins localized in the nucleus but showed no transcriptional activation activity as a monomer. Subsequently, it was found that the heterologous expression of the ZmBES1/BZR1-3 and ZmBES1/BZR1-9 genes in Arabidopsis decreased drought tolerance, respectively. The transgenic lines showed a more serious wilting phenotype, shorter root length, lower fresh weight, and higher relative electrolyte leakage (REL) and malondialdehyde (MDA) content compared to the control under drought stress. The RNA-sequencing data showed that the 70.67% and 93.27% differentially expressed genes (DEGs) were significantly down-regulated in ZmBES1/BZR1-3 and ZmBES1/BZR1-9 transgenic Arabidopsis, respectively. The DEGs of ZmBES1/BZR1-3 gene’s expressing lines were mainly associated with oxidative stress response and amino acid metabolic process and enriched in phenylpropanoid biosynthesis and protein processing in the endoplasmic reticulum. But the DEGs of the ZmBES1/BZR1-9 gene’s expressing lines were predominantly annotated with water deprivation, extracellular stimuli, and jasmonic acid and enriched in phenylpropanoid biosynthesis and plant hormone signal transduction. Moreover, ZmBES1/BZR1-9 increased stomatal aperture in transgenic Arabidopsis under drought stress. This study indicates that ZmBES1/BZR1-3 and ZmBES1/BZR1-9 negatively regulate drought tolerance via different pathways in transgenic Arabidopsis, and it provides insights into the underlying the function of BES1/BZR1s in crops.
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影响因子：
5.6
作者：
Reyes-Impellizzeri S;Moreno AA
通讯作者：
Moreno AA
DOI：
10.15252/embj.2021108664
发表时间：
2022-02-01
期刊：
The EMBO journal
影响因子：
--
作者：
Albertos P;Dündar G;Schenk P;Carrera S;Cavelius P;Sieberer T;Poppenberger B
通讯作者：
Poppenberger B
ZmWRKY79正向调节玉米植物抗毒素生物合成基因表达并参与应激反应
影响因子：
6.9
作者：
Fu, Jingye;Liu, Qin;Wang, Qiang
通讯作者：
Wang, Qiang
影响因子：
3
作者：
Galstyan, Anahit;Nemhauser, Jennifer L.
通讯作者：
Nemhauser, Jennifer L.
影响因子：
27.5
作者：
Amtmann, Anna
通讯作者：
Amtmann, Anna