Graded spikes differentially signal neurotransmitter input in cerebrospinal fluid contacting neurons of the mouse spinal cord.
Graded spikes differentially signal neurotransmitter input in cerebrospinal fluid contacting neurons of the mouse spinal cord.
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DOI：
10.1016/j.isci.2022.105914
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发表时间：
2023-01-20
期刊：
影响因子：
5.8
通讯作者：
Johnston, Jamie
中科院分区：
文献类型：
--
作者：
Johnson, Emily;Clark, Marilyn;Oncul, Merve;Pantiru, Andreea;MacLean, Claudia;Deuchars, Jim;Deuchars, Susan A.;Johnston, Jamie
动作电位及其全有或全无的性质是神经通信的基础。通常，一旦电压激活的Na+通道被激活，动作电位就被启动，并且它们的快速激活和失活动力学引起动作电位的全或无性质。在这里，我们表明，接触脑脊液神经元（CSFcNs）周围的小鼠脊髓中央管采用不同的策略。CSFcN不是使用电压激活的Na+通道来产生二进制尖峰，而是使用两种不同类型的电压激活的Ca 2+通道，从而实现不同幅度的尖峰。T型Ca 2+通道产生小幅度的尖峰，而较大幅度的尖峰需要高电压激活的Cd 2+敏感的Ca 2+通道。我们表明，这些不同的振幅尖峰信号可以从不同的发射系统输入;嘌呤输入引起较小的T型依赖的尖峰，而胆碱能输入引起较大的尖峰，不依赖于T型通道。因此，到CSFcN的不同突触输入可以通过尖峰幅度来发信号。CSFcN动作电位由两种类型的Ca 2+通道介导。差异募集可以产生不同幅度的动作电位。CSFcN可以用尖峰幅度代码发出其神经递质输入的信号。
The action potential and its all-or-none nature is fundamental to neural communication. Canonically, the action potential is initiated once voltage-activated Na+ channels are activated, and their rapid kinetics of activation and inactivation give rise to the action potential’s all-or-none nature. Here we demonstrate that cerebrospinal fluid contacting neurons (CSFcNs) surrounding the central canal of the mouse spinal cord employ a different strategy. Rather than using voltage-activated Na+ channels to generate binary spikes, CSFcNs use two different types of voltage-activated Ca2+ channel, enabling spikes of different amplitude. T-type Ca2+ channels generate small amplitude spikes, whereas larger amplitude spikes require high voltage-activated Cd2+-sensitive Ca2+ channels. We demonstrate that these different amplitude spikes can signal input from different transmitter systems; purinergic inputs evoke smaller T-type dependent spikes whereas cholinergic inputs evoke larger spikes that do not rely on T-type channels. Different synaptic inputs to CSFcNs can therefore be signaled by the spike amplitude. CSFcN action potentials are mediated by two types of Ca2+ channels Differential recruitment can generate action potentials of varying amplitude CSFcNs can signal their neurotransmitter input with a spike amplitude code CSFcNs are mechanosensitive excited by placement of a recording electrode Molecular neuroscience; Systems neuroscience; Cellular neuroscience.
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牛肾上腺筋膜细胞中电压门控瞬时电流。 I. T型Ca2+电流。
影响因子：
3.8
作者：
Mlinar, B;Biagi, B A;Enyeart, J J
通讯作者：
Enyeart, J J
影响因子：
5.3
作者：
Fekete, Adam;Johnston, Jamie;Delaney, Kerry R.
通讯作者：
Delaney, Kerry R.
影响因子：
5.2
作者：
Corns, Laura F.;Atkinson, Lucy;Deuchars, Susan A.
通讯作者：
Deuchars, Susan A.
DOI：
10.1073/pnas.93.18.9921
发表时间：
1996-09-03
影响因子：
11.1
作者：
Buzsaki, G;Penttonen, M;Bragin, A
通讯作者：
Bragin, A
影响因子：
1.2
作者：
Holt, GR;Koch, C
通讯作者：
Koch, C