HKOCl-3: a fluorescent hypochlorous acid probe for live-cell and in vivo imaging and quantitative application in flow cytometry and a 96-well microplate assay.
HKOCl-3: a fluorescent hypochlorous acid probe for live-cell and in vivo imaging and quantitative application in flow cytometry and a 96-well microplate assay.
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DOI：
10.1039/c5sc03855c
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发表时间：
2016-03-01
期刊：
影响因子：
8.4
通讯作者：
Yang D
中科院分区：
文献类型：
--
作者：
Hu JJ;Wong NK;Lu MY;Chen X;Ye S;Zhao AQ;Gao P;Yi-Tsun Kao R;Shen J;Yang D
我们描述了一种超选择性和超灵敏度的HOCI荧光探针HKOCl-3活细胞和体内成像和定量应用。超选择性和超灵敏度的次氯酸（HOCl），最了解的活性氧（ROS）之一，是迫切需要解开的重要生物过程中，如发育和先天免疫的HOCl功能。基于2，6-二氯苯酚选择性氧化O-脱芳基生成HOCl的反应，我们开发了一种新型的HOCl荧光探针HKOCl-3，该探针具有超选择性、超灵敏度和快速开启响应。HKOCl-3用于内源性HOCl检测和成像的功能稳健性已经在多种类型的吞噬细胞和活的完整斑马鱼胚胎的体内成像中进行了彻底的检查。此外，HKOCl-3已成功地应用于检测内源性HOCl的96孔微孔板测定和流式细胞术。因此，HKOCl-3作为一种多功能的分子工具，具有很大的前景，可以在多种检测模式中对HOCl生物学和ROS相关疾病进行创新研究。
We describe an ultra-selective and ultra-sensitive HOCI fluorescent probe HKOCl-3 for live-cell and in vivo imaging and quantitative applications. Ultra-selective and ultra-sensitive probes for hypochlorous acid (HOCl), one of the most poorly understood reactive oxygen species (ROS), are urgently needed to unravel the HOCl functions in important biological processes such as development and innate immunity. Based on a selective oxidative O-dearylation reaction of 2,6-dichlorophenol toward HOCl over other reactive oxygen species, we have developed a novel fluorescent probe HKOCl-3 for HOCl detection with ultra-selectivity, ultra-sensitivity and a rapid turn-on response. The functional robustness of HKOCl-3 for endogenous HOCl detection and imaging has been thoroughly scrutinized in multiple types of phagocytes and in vivo imaging of live intact zebrafish embryos. Furthermore, HKOCl-3 has been successfully applied to the detection of endogenous HOCl by a 96-well microplate assay and flow cytometry. Therefore, HKOCl-3 holds great promise as a versatile molecular tool that enables innovative investigation of HOCl biology and ROS-related diseases in multiple detection modalities.
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影响因子：
20.3
作者：
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通讯作者：
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影响因子：
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