A photoperiod-regulating gene CONSTANS is correlated to lipid biosynthesis in Chlamydomonas reinhardtii.
A photoperiod-regulating gene CONSTANS is correlated to lipid biosynthesis in Chlamydomonas reinhardtii.
复制标题
光周期调节基因 CONSTANS 与莱茵衣藻的脂质生物合成相关。
DOI：
10.1155/2015/715020
复制
发表时间：
2015
影响因子：
--
通讯作者：
Fei X
中科院分区：
文献类型：
--
作者：
Deng X;Fan X;Li P;Fei X
背景。脂质生物合成的调控在光合真核细胞中是必不可少的。目前尚无脂质代谢途径的调控基因报道。植物CONSTANS （CO）基因通过参与光周期和生物钟调控开花。除了调节光周期外，衣藻CO基因还调节淀粉含量。结果。本研究结果显示，在HSM-S条件下，短日照条件下的细胞比长日照条件下的细胞积累更多的脂质。通过RNA干扰使CrCO基因沉默，导致脂质含量增加，甘油三酯（TAG）水平增加24.5%。CrCO RNAi菌株在短日照条件下比在长日照条件下积累更多的脂质。CrCO表达的降低导致TAG生物合成相关基因DGAT2、PAP2、PDAT3的表达增加，而CIS和FBP1基因在CrCO表达被抑制时mRNA表达减少。另一方面，CrCO过表达导致脂质含量和TAG水平下降。结论。本研究结果揭示了CrCO基因与衣藻脂质代谢之间的关系，提示通过抑制CrCO在微藻中的表达来增加油脂是可行的。
Background. The regulation of lipid biosynthesis is essential in photosynthetic eukaryotic cells. Thus far, no regulatory genes have been reported in the lipid metabolism pathway. Plant CONSTANS (CO) gene regulates blooming by participating in photoperiod and biological clock. Apart from regulating photoperiod, the Chlamydomonas CO gene also regulates starch content. Results. In this study, the results showed that, under HSM-S condition, cells accumulated more lipids at short-day conditions than at long-day conditions. The silencing of the CrCO gene via RNA interference resulted in an increase in lipid content and an increase in triacylglyceride (TAG) level by 24.5%. CrCO RNAi strains accumulated more lipids at short-day conditions than at long-day conditions. The decrease in CrCO expression resulted in the increased expression of TAG biosynthesis-related genes, such as DGAT2, PAP2, and PDAT3, whereas CIS and FBP1 genes showed a decrease in their mRNA when the CrCO expression was suppressed. On the other hand, the overexpression of CrCO resulted in the decrease in lipid content and TAG level. Conclusions. The results of this study revealed a relationship between CrCO gene and lipid metabolism in Chlamydomonas, suggesting that increasing oil by suppressing CrCO expression in microalgae is feasible.
登录
查看更多内容
莱茵衣藻pepc2基因mRNA丰度与油脂含量呈负相关
影响因子：
6
作者：
Deng, Xiaodong;Li, Yajun;Fei, Xiaowen
通讯作者：
Fei, Xiaowen
影响因子：
2.2
作者：
Chen, Wei;Zhang, Chengwu;Hu, Qiang
通讯作者：
Hu, Qiang
影响因子：
2.2
作者：
Gao, Chunfang;Xiong, Wei;Wu, Qingyu
通讯作者：
Wu, Qingyu
影响因子：
7.2
作者：
Ben-Naim, O;Eshed, R;Lifschitz, E
通讯作者：
Lifschitz, E
影响因子：
11.6
作者：
Datta, Sourav;Johansson, Henrik;Holm, Magnus
通讯作者：
Holm, Magnus