Circulating Tumor Cells from Enumeration to Analysis: Current Challenges and Future Opportunities.
Circulating Tumor Cells from Enumeration to Analysis: Current Challenges and Future Opportunities.
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DOI：
10.3390/cancers13112723
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发表时间：
2021-05-31
期刊：
影响因子：
5.2
通讯作者：
Cote RJ
中科院分区：
文献类型：
--
作者：
Yang YP;Giret TM;Cote RJ
每毫升血液中估计有 1-10 个循环肿瘤细胞 (CTC)，与白细胞（几百万）或红细胞（数十亿）相比，循环肿瘤细胞 (CTC) 极其罕见。鉴于 CTC 在转移中的关键作用，CTC 作为癌症诊断、预后和治疗反应监测的生物标志物具有巨大的潜力。现在人们正在努力通过分子和功能分析来更精确地表征 CTC，将 CTC 的工作范围从诊断和预后之一扩展到现在包括使用 CTC 专门针对癌症和发现治疗解决方案，从而确立 CTC 在精准医学中的关键地位。本文总结了有关 CTC 分离技术的最新知识，并讨论了不同类型下游分析方法的转化优势，包括单 CTC 分析、CTC 体外扩增以及 CTC 相关细胞的表征。循环肿瘤细胞（CTC）已被认为是远处转移的主要贡献者。它们作为转移种子的独特作用使它们成为早期癌症诊断和预后以及治疗反应监测的循环中的潜在标记。过去十年，研究人员主要致力于开发分离技术以提高CTC的回收率和纯度。这些开发的技术显着提高了 CTC 的检测灵敏度和计数准确性。目前，人们在全面的分子表征、CTC 的离体扩增以及了解循环中 CTC 与其相关细胞（例如免疫细胞和基质细胞）之间的相互作用方面做出了重大努力。在这篇综述中，我们简要总结了现有的 CTC 分离技术，并特别关注 CTC 下游分析的进展及其在精准医学中的潜在应用。我们还讨论了其临床应用中当前的挑战和未来的机遇。
With estimated numbers of 1–10 per mL of blood, circulating tumor cells (CTCs) are extremely rare compared to white (a few million) or red (billions) blood cells. Given their critical role in metastasis, CTCs have enormous potential as a biomarker for cancer diagnosis, prognosis, and monitoring of treatment response. There are now efforts to characterize CTCs more precisely through molecular and functional analysis, expanding the CTC effort from one of diagnosis and prognosis to now include the use of CTCs to specifically target cancers and discover therapeutic solutions, establishing CTCs as critical in precision medicine. This article summarizes current knowledge about CTC isolation technologies and discusses the translational benefits of different types of downstream analysis approaches, including single-CTC analysis, ex vivo expansion of CTCs, and characterization of CTC-associated cells. Circulating tumor cells (CTCs) have been recognized as a major contributor to distant metastasis. Their unique role as metastatic seeds renders them a potential marker in the circulation for early cancer diagnosis and prognosis as well as monitoring of therapeutic response. In the past decade, researchers mainly focused on the development of isolation techniques for improving the recovery rate and purity of CTCs. These developed techniques have significantly increased the detection sensitivity and enumeration accuracy of CTCs. Currently, significant efforts have been made toward comprehensive molecular characterization, ex vivo expansion of CTCs, and understanding the interactions between CTCs and their associated cells (e.g., immune cells and stromal cells) in the circulation. In this review, we briefly summarize existing CTC isolation technologies and specifically focus on advances in downstream analysis of CTCs and their potential applications in precision medicine. We also discuss the current challenges and future opportunities in their clinical utilization.
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影响因子：
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作者：
Babayan A;Alawi M;Gormley M;Müller V;Wikman H;McMullin RP;Smirnov DA;Li W;Geffken M;Pantel K;Joosse SA
通讯作者：
Joosse SA
DOI：
10.1186/s13058-016-0740-2
发表时间：
2016-08-11
期刊：
Breast cancer research : BCR
影响因子：
--
作者：
Bussard KM;Mutkus L;Stumpf K;Gomez-Manzano C;Marini FC
通讯作者：
Marini FC
DOI：
10.1073/pnas.1524448113
发表时间：
2016-05-03
影响因子：
11.1
作者：
Au, Sam H.;Storey, Brian D.;Toner, Mehmet
通讯作者：
Toner, Mehmet
DOI：
10.1073/pnas.92.3.860
发表时间：
1995-01-31
影响因子：
11.1
作者：
BECKER, FF;WANG, XB;GASCOYNE, PRC
通讯作者：
GASCOYNE, PRC
DOI：
10.1007/978-3-642-28160-0_6
发表时间：
2012-01-01
期刊：
Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer
影响因子：
--
作者：
Alix-Panabieres, Catherine
通讯作者：
Alix-Panabieres, Catherine