Local Delivery of Autologous Platelet in Collagen Matrix Synergistically Stimulated In-situ Articular Cartilage Repair
Local Delivery of Autologous Platelet in Collagen Matrix Synergistically Stimulated In-situ Articular Cartilage Repair
复制标题
胶原基质中自体血小板的局部输送协同刺激原位关节软骨修复
DOI：
10.1007/978-3-540-92841-6_317
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发表时间：
2009
影响因子：
3.3
通讯作者：
Jiang, Yang Zi
中科院分区：
文献类型：
--
作者：
Qi, Yi Ying;Ouyang, Hong Wei;Wang, Lin Lin;Hieu, Nguyen Thi Minh;Chen, Xiao;Cai, Hong Xin;Jiang, Yang Zi
目的：微骨折术是一种常规的软骨下骨骨髓松解原位修复软骨的方法。然而，它只能治疗小的软骨缺损（< 2 cm 2）。本研究旨在探讨自体富血小板血浆（PRP）在胶原基质中的移植是否能协同促进原位骨髓启动的软骨修复。实验设计：选用雄性新西兰白色兔髌骨沟全层软骨缺损（直径= 4 mm，厚度=3 mm）作为原位软骨修复模型。分别用双层胶原支架（II组）、PRP+双层胶原支架（III组）和未处理组（I组）治疗11例。分别于术后6周和12周处死动物。结果：结果显示，在6周和12周时，Ⅲ组软骨组织数量最多，修复的软骨缺损面积较大。结果：自体PRP和双层胶原基质可协同促进软骨组织的形成，修复后软骨组织的弹性模量明显高于I组（p<0.05），且修复后软骨组织的组织学评分和糖胺聚糖含量均高于I组（p<0.05）。研究结果表明，微骨折与胶原基质中的PRP的组合可以修复目前需要复杂的自体软骨细胞植入（ACI）或骨软骨移植的大于2 cm 2的某些类型的软骨缺损。韧带和肌腱组织工程支架的研究已经开展了很多。然而，同时具有最佳强度、多孔结构和生物相容性微环境的支架还有待开发。本研究旨在通过蚕丝材料、编织结构和胶原基质的协同结合，设计一种新型实用的韧带支架。
Objective: Microfracture is a routine therapy to release bone marrow from subchondral bone for in-situ cartilage repair. However, it can only treat small cartilage defects (< 2 cm2). The study aims to investigate whether autologous platelet rich plasma (PRP) transplantation in collagen matrix can synergistically improve the in-situ bone marrow-initiated cartilage repair.Experimental design: Full-thickness cartilage defects (diameter = 4 mm, thickness =3 mm) in the patellar grooves of male New Zealand White rabbits were chosen as model of insitu cartilage repair. They were treated with bilayer collagen scaffold (group II), PRP and bilayer collagen scaffold (group III) and untreated (group I) respectively (n=11). The rabbits were sacrificed at 6 and 12 weeks after operation. The repaired tissues were processed for histology and for mechanical test.Results: The results showed that at both 6 and 12 weeks, group III had the largest amounts of cartilage tissue, which restored larger surface area of the cartilage defects. Moreover, group III had higher histological scores and more glycosaminoglycans (GAGs) content than those in other two groups (p<0.05).The Young’s modulus of the repaired tissue in group II and group III were significantly higher than that of group I (p<0.05).Conclusion: Autologous PRP and bilayer collagen matrix synergistically stimulated the formation of cartilage tissues. The findings implicated that the combination of microfracture with PRP in collagen matrix may repair certain type of cartilage defects larger than 2 cm2 which currently require the complex autologous chondrocyte implantation (ACI) or osteochondral grafting. number of researches on ligament and tendon tissue engineering scaffold have been carried out. However, a scaffold which simultaneously possesses optimal strength, porous structure and biocompatible microenvironment is yet to be developed. This study aims to design a new practical ligament scaffold by synergic incorporation of silk material, knitting structure and collagen matrix.
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影响因子：
4.7
作者：
DZIOBA R B
通讯作者：
DZIOBA R B
DOI：
10.2106/00004623-200300002-00007
发表时间：
2003-01-01
影响因子：
5.3
作者：
Mainil-Varlet, P;Aigner, T;Stauffer, E
通讯作者：
Stauffer, E
影响因子：
4.1
作者：
C. Charalambous
通讯作者：
C. Charalambous
影响因子：
14
作者：
Buma, P;Pieper, JS;van Kuppevelt, TH
通讯作者：
van Kuppevelt, TH
DOI：
10.2519/jospt.1998.28.4.192
发表时间：
1998-10-01
影响因子：
6.1
作者：
Buckwalter, JA
通讯作者：
Buckwalter, JA