Structures of new phenolics isolated from licorice, and the effectiveness of licorice phenolics on vancomycin-resistant Enterococci.
Structures of new phenolics isolated from licorice, and the effectiveness of licorice phenolics on vancomycin-resistant Enterococci.
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DOI：
10.3390/molecules190913027
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发表时间：
2014-08-25
期刊：
影响因子：
--
通讯作者：
Hatano T
中科院分区：
文献类型：
--
作者：
Eerdunbayaer;Orabi MA;Aoyama H;Kuroda T;Hatano T
甘草是甘草属植物的地下部分，在亚洲和西方国家被广泛用作传统药物和食品添加剂。通过对乌拉尔甘草根和匍匐茎化学成分的研究，在14个已知化合物的基础上，又分离出两个新的酚类化合物。结构研究包括光谱和简单的化学衍生化，表明这两个新化合物具有2-芳基-3-甲基苯并呋喃结构。本研究中获得的甘草酚类物质对万古霉素耐药菌株屎肠球菌FN-1和粪肠球菌NCTC 12201的有效性的检查显示，甘草酸对两种大肠杆菌显示出最有效的抗菌作用。粪肠球菌E.最小抑菌浓度（MIC）为1.9 × 10−5 M。8-（γ，γ-Dimethylallyl）-wighteone、isoangustone A、3 '-（γ，γ-dimethylallyl）-kievitone、glyasperin C和一种新的3-甲基-2-苯基苯并呋喃（称为neoglycybenzofuran）也显示出有效的抗万古霉素耐药肠球菌作用（对大肠杆菌的MIC为1.9 × 10−5-4.5 × 10−5 M）。屎肠球菌和粪便）。为评价天然抗菌资源的质量控制，研究了提取物中酚类化合物的高效液相色谱同时检测条件，并对提取物中几种主要酚类化合物进行了定量测定。结果显示，所检查材料中主要酚类物质的EtOAc浸提液的单个含量为0.08%-0.57% w/w。
Licorice, which is the underground part of Glycyrrhiza species, has been used widely in Asian and Western countries as a traditional medicine and as a food additive. Our continuous investigation on the constituents of roots and stolons of Glycyrrhiza uralensis led to the isolation of two new phenolics, in addition to 14 known compounds. Structural studies including spectroscopic and simple chemical derivatizations revealed that both of the new compounds had 2-aryl-3-methylbenzofuran structures. An examination of the effectiveness of licorice phenolics obtained in this study on vancomycin-resistant strains Enterococcus faecium FN-1 and Enterococcus faecalis NCTC12201 revealed that licoricidin showed the most potent antibacterial effects against both of E. faecalis and E. faecium with a minimum inhibitory concentration (MIC) of 1.9 × 10−5 M. 8-(γ,γ-Dimethylallyl)-wighteone, isoangustone A, 3'-(γ,γ-dimethylallyl)-kievitone, glyasperin C, and one of the new 3-methyl-2-phenylbenzofuran named neoglycybenzofuran also showed potent anti-vancomycin-resistant Enterococci effects (MIC 1.9 × 10−5–4.5 × 10−5 M for E. faecium and E. faecalis). The HPLC condition for simultaneous detection of the phenolics in the extract was investigated to assess the quality control of the natural antibacterial resource, and quantitative estimation of several major phenolics in the extract with the established HPLC condition was also performed. The results showed individual contents of 0.08%–0.57% w/w of EtOAc extract for the major phenolics in the materials examined.
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影响因子：
3.1
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影响因子：
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作者：
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