Immunostimulatory CpG oligodeoxynucleotides enhance the immune response to vaccine strategies involving granulocyte-macrophage colony-stimulating factor.
Immunostimulatory CpG oligodeoxynucleotides enhance the immune response to vaccine strategies involving granulocyte-macrophage colony-stimulating factor.
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免疫刺激性 CpG 寡脱氧核苷酸可增强对涉及粒细胞-巨噬细胞集落刺激因子的疫苗策略的免疫反应。
DOI：
10.1182/blood.v92.10.3730.422k20_3730_3736
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发表时间：
1998
期刊：
影响因子：
20.3
通讯作者：
G. Weiner
中科院分区：
文献类型：
--
作者：
Hsin;Sally E. Newbrough;Sudershan K. Bhatia;C. Dahle;A. Krieg;G. Weiner
含有CpG基序的免疫刺激寡脱氧核苷酸（CpG ODN）可以激活多种免疫细胞亚群并诱导多种细胞因子的产生。先前的研究表明，CpG ODN和粒细胞-巨噬细胞集落刺激因子（GM-CSF）都可以作为有效的疫苗佐剂。我们使用38C13小鼠淋巴瘤系统来评估对这两种佐剂组合的免疫反应。使用抗原、CpG ODN和可溶性GM-CSF的免疫增强了抗原特异性抗体的产生，并将产生转向IgG2a同型，表明TH1应答增强。这种效果在CpG ODN和抗原/GM-CSF融合蛋白重复免疫后最为明显。在肿瘤接种前3天用CpG ODN和抗原/GM-CSF融合蛋白单次免疫可抑制肿瘤生长。CpG ODN增强了骨髓源树突状细胞产生白细胞介素-12，并增加了主要组织相容性复合体I类和II类分子的表达，特别是当细胞被抗原/GM-CSF融合蛋白脉冲时。我们得出结论，CpG ODN与涉及GM-CSF的策略联合使用增强了对抗原的免疫应答，并将应答转向TH1应答，这种方法值得在肿瘤免疫方法和其他需要抗原特异性TH1应答的条件下进一步评估。
Immunostimulatory oligodeoxynucleotides containing the CpG motif (CpG ODN) can activate various immune cell subsets and induce production of a number of cytokines. Prior studies have demonstrated that both CpG ODN and granulocyte-macrophage colony-stimulating factor (GM-CSF) can serve as potent vaccine adjuvants. We used the 38C13 murine lymphoma system to evaluate the immune response to a combination of these two adjuvants. Immunization using antigen, CpG ODN, and soluble GM-CSF enhanced production of antigen-specific antibody and shifted production towards the IgG2a isotype, suggesting an enhanced TH1 response. This effect was most pronounced after repeat immunizations with CpG ODN and antigen/GM-CSF fusion protein. A single immunization with CpG ODN and antigen/GM-CSF fusion protein 3 days before tumor inoculation prevented tumor growth. CpG ODN enhanced the production of interleukin-12 by bone marrow-derived dendritic cells and increased expression of major histocompatibility complex class I and class II molecules, particularly when cells were pulsed with antigen/GM-CSF fusion protein. We conclude that the use of CpG ODN in combination with strategies involving GM-CSF enhances the immune response to antigen and shifts the response towards a TH1 response and that this approach deserves further evaluation in tumor immunization approaches and other conditions in which an antigen-specific TH1 response is desirable.
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DOI：
10.1073/pnas.93.7.2879
发表时间：
1996-04-02
影响因子：
11.1
作者：
Klinman, DM;Yi, AK;Krieg, AM
通讯作者：
Krieg, AM
影响因子：
20.3
作者：
Wooldridge, JE;Ballas, Z;Weiner, GJ
通讯作者：
Weiner, GJ
影响因子：
4.4
作者：
J. Messina;G. Gilkeson;D. Pisetsky
通讯作者：
J. Messina;G. Gilkeson;D. Pisetsky
影响因子：
4.4
作者：
M. Kaminski;K. Kitamura;D. Maloney;R. Levy
通讯作者：
M. Kaminski;K. Kitamura;D. Maloney;R. Levy
IFN-γ 响应细菌 DNA 和寡脱氧核苷酸中的 CpG 基序，促进 IL-6 和 IgM 分泌。
DOI：
--
发表时间：
1996
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Yi,AK;Chace,JH;Cowdery,JS;Krieg,AM
通讯作者：
Krieg,AM